The Sakaguchi Laboratory of Developmental Biology Department of Systems Medicine

2025

Electronic Patient-Reported Outcome System Implementation in Outpatient Cardiovascular Care: A Randomized Clinical Trial

Yamashita, S., Katsumata, Y., Kohsaka, S., Kitakata, H., Shiraishi, Y., Yamaoka, K., Muramoto, Y., Ono, T., Shoji, S., Yagyu, K., Oginosawa, Y., Kataoka, M., Hashimoto, M., Ko, S. B. H., Kitagawa, Y. & Jinzaki, M., 2025 Jan 2, In: JAMA network open. e2454084.

Research output: Contribution to journal › Article › peer-review

Open Access

1 Citation (Scopus)

2021

Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis

Horisawa-Takada, Y., Kodera, C., Takemoto, K., Sakashita, A., Horisawa, K., Maeda, R., Shimada, R., Usuki, S., Fujimura, S., Tani, N., Matsuura, K., Akiyama, T., Suzuki, A., Niwa, H., Tachibana, M., Ohba, T., Katabuchi, H., Namekawa, S. H., Araki, K. & Ishiguro, K. I., 2021 Dec 1, In: Nature communications. 12, 1, 3184.

Research output: Contribution to journal › Article › peer-review

Open Access

24 Citations (Scopus)

Synthetic mRNA-based differentiation method enables early detection of Parkinson's phenotypes in neurons derived from Gaucher disease-induced pluripotent stem cells

Akiyama, T., Sato, S., Ko, S. B. H., Sano, O., Sato, S., Saito, M., Nagai, H., Ko, M. S. H. & Iwata, H., 2021 Apr, In: Stem Cells Translational Medicine. 10, 4, p. 572-581 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Citations (Scopus)

Unbiased, comprehensive analysis of Japanese health checkup data reveals a protective effect of light to moderate alcohol consumption on lung function

Makino, K., Shimizu-Hirota, R., Goda, N., Hashimoto, M., Kawada, I., Kashiwagi, K., Hirota, Y., Itoh, H., Jinzaki, M., Iwao, Y., Ko, M., Ko, S. & Takaishi, H., 2021 Dec, In: Scientific reports. 11, 1, 15954.

Research output: Contribution to journal › Article › peer-review

Open Access

5 Citations (Scopus)

2020

Fatty Acid Synthesis Is Indispensable for Survival of Human Pluripotent Stem Cells

Tanosaki, S., Tohyama, S., Fujita, J., Someya, S., Hishiki, T., Matsuura, T., Nakanishi, H., Ohto-Nakanishi, T., Akiyama, T., Morita, Y., Kishino, Y., Okada, M., Tani, H., Soma, Y., Nakajima, K., Kanazawa, H., Sugimoto, M., Ko, M. S. H., Suematsu, M. & Fukuda, K., 2020 Sept 25, In: iScience. 23, 9, 101535.

Research output: Contribution to journal › Article › peer-review

Open Access

63 Citations (Scopus)

Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors

Nakatake, Y., Ko, S. B. H., Sharov, A. A., Wakabayashi, S., Murakami, M., Sakota, M., Chikazawa, N., Ookura, C., Sato, S., Ito, N., Ishikawa-Hirayama, M., Mak, S. S., Jakt, L. M., Ueno, T., Hiratsuka, K., Matsushita, M., Goparaju, S. K., Akiyama, T., Ishiguro, K. I. & Oda, M. & 7 others, Gouda, N., Umezawa, A., Akutsu, H., Nishimura, K., Matoba, R., Ohara, O. & Ko, M. S. H., 2020 May 19, In: Cell Reports. 31, 7, 107655.

Research output: Contribution to journal › Article › peer-review

Open Access

20 Citations (Scopus)

MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells

Ishiguro, K. I., Matsuura, K., Tani, N., Takeda, N., Usuki, S., Yamane, M., Sugimoto, M., Fujimura, S., Hosokawa, M., Chuma, S., Ko, M. S. H., Araki, K. & Niwa, H., 2020 Feb 24, In: Developmental Cell. 52, 4, p. 429-445.e10

Research output: Contribution to journal › Article › peer-review

Open Access

123 Citations (Scopus)

2019

Induced Pluripotent Stem Cells Reprogrammed with Three Inhibitors Show Accelerated Differentiation Potentials with High Levels of 2-Cell Stage Marker Expression

Nishihara, K., Shiga, T., Nakamura, E., Akiyama, T., Sasaki, T., Suzuki, S., Ko, M. S. H., Tada, N., Okano, H. & Akamatsu, W., 2019 Feb 12, In: Stem cell reports. 12, 2, p. 305-318 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Citations (Scopus)

Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors

Hiratsuka, K., Monkawa, T., Akiyama, T., Nakatake, Y., Oda, M., Goparaju, S. K., Kimura, H., Chikazawa-Nohtomi, N., Sato, S., Ishiguro, K., Yamaguchi, S., Suzuki, S., Morizane, R., Ko, S. B. H., Itoh, H. & Ko, M. S. H., 2019 Dec 1, In: Scientific reports. 9, 1, 913.

Research output: Contribution to journal › Article › peer-review

Open Access

42 Citations (Scopus)

In vitro modeling of the bipolar disorder and schizophrenia using patient-derived induced pluripotent stem cells with copy number variations of PCDH15 and RELN

Ishii, T., Ishikawa, M., Fujimori, K., Maeda, T., Kushima, I., Arioka, Y., Mori, D., Nakatake, Y., Yamagata, B., Nio, S., Kato, T. A., Yang, N., Wernig, M., Kanba, S., Mimura, M., Ozaki, N. & Okano, H., 2019, In: eNeuro. 6, 5, ENEURO.0403-18.2019.

Research output: Contribution to journal › Article › peer-review

Open Access

53 Citations (Scopus)

2018

Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing

Akiyama, T., Sato, S., Chikazawa-Nohtomi, N., Soma, A., Kimura, H., Wakabayashi, S., Ko, S. B. H. & Ko, M. S. H., 2018 Dec 1, In: Scientific reports. 8, 1, 1189.

Research output: Contribution to journal › Article › peer-review

Open Access

25 Citations (Scopus)

Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors 10 Technology 1004 Medical Biotechnology

Ida, H., Akiyama, T., Ishiguro, K., Goparaju, S. K., Nakatake, Y., Chikazawa-Nohtomi, N., Sato, S., Kimura, H., Yokoyama, Y., Nagino, M., Ko, M. S. H. & Ko, S. B. H., 2018 Oct 25, In: Stem Cell Research and Therapy. 9, 1, 277.

Research output: Contribution to journal › Article › peer-review

Open Access

12 Citations (Scopus)

The pathogenesis linked to coenzyme Q10 insufficiency in iPSC-derived neurons from patients with multiple-system atrophy

Nakamoto, F. K., Okamoto, S., Mitsui, J., Sone, T., Ishikawa, M., Yamamoto, Y., Kanegae, Y., Nakatake, Y., Imaizumi, K., Ishiura, H., Tsuji, S. & Okano, H., 2018 Dec 1, In: Scientific reports. 8, 1, 14215.

Research output: Contribution to journal › Article › peer-review

Open Access

53 Citations (Scopus)

2017

Bicarbonate-rich fluid secretion predicted by a computational model of guinea-pig pancreatic duct epithelium

Yamaguchi, M., Steward, M. C., Smallbone, K., Sohma, Y., Yamamoto, A., Ko, S. B. H., Kondo, T. & Ishiguro, H., 2017 Mar 15, In: Journal of Physiology. 595, 6, p. 1947-1972 26 p.

Research output: Contribution to journal › Article › peer-review

Open Access

25 Citations (Scopus)

Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells

Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. B. H. & Ko, M. S. H., 2017, In: Stem Cells International. 2017, 7215010.

Research output: Contribution to journal › Review article › peer-review

Open Access

5 Citations (Scopus)

Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos

Ishiguro, K. I., Nakatake, Y., Chikazawa-Nohtomi, N., Kimura, H., Akiyama, T., Oda, M., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 1, In: In Vitro Cellular and Developmental Biology - Animal. 53, 2, p. 179-190 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

12 Citations (Scopus)

Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells

Hirayama, M., Ko, S. B. H., Kawakita, T., Akiyama, T., Goparaju, S. K., Soma, A., Nakatake, Y., Sakota, M., Chikazawa-Nohtomi, N., Shimmura, S., Tsubota, K. & Ko, M. S. H., 2017 Dec 1, In: npj Aging and Mechanisms of Disease. 3, 1, 1.

Research output: Contribution to journal › Article › peer-review

Open Access

28 Citations (Scopus)

Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors

Matsushita, M., Nakatake, Y., Arai, I., Ibata, K., Kohda, K., Goparaju, S. K., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. B. H., Kanai, T., Yuzaki, M. & Ko, M. S. H., 2017 Aug 19, In: Biochemical and Biophysical Research Communications. 490, 2, p. 296-301 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

25 Citations (Scopus)

Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Goparaju, S. K., Kohda, K., Ibata, K., Soma, A., Nakatake, Y., Akiyama, T., Wakabayashi, S., Matsushita, M., Sakota, M., Kimura, H., Yuzaki, M., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 13, In: Scientific reports. 7, 42367.

Research output: Contribution to journal › Article › peer-review

Open Access

73 Citations (Scopus)

Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

Arai, Y., Takahashi, D., Asano, K., Tanaka, M., Oda, M., Ko, S. B. H., Ko, M. S. H., Mandai, S., Nomura, N., Rai, T., Uchida, S. & Sohara, E., 2017, In: Scientific reports. 7, 46580.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Citations (Scopus)

SCODE: An efficient regulatory network inference algorithm from single-cell RNA-Seq during differentiation

Matsumoto, H., Kiryu, H., Furusawa, C., Ko, M. S. H., Ko, S. B. H., Gouda, N., Hayashi, T. & Nikaido, I., 2017 Aug 1, In: Bioinformatics. 33, 15, p. 2314-2321 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

290 Citations (Scopus)

Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis

Ishiguro, K. I., Monti, M., Akiyama, T., Kimura, H., Chikazawa-Nohtomi, N., Sakota, M., Sato, S., Redi, C. A., Ko, S. B. H. & Ko, M. S. H., 2017 Feb 1, In: In Vitro Cellular and Developmental Biology - Animal. 53, 2, p. 167-178 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

12 Citations (Scopus)

2016

Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment

Sharova, L. V., Sharov, A. A., Piao, Y., Stagg, C. A., Amano, T., Qian, Y., Dudekula, D., Schlessinger, D. & Ko, M. S. H., 2016 May 1, In: In Vitro Cellular and Developmental Biology - Animal. 52, 5, p. 616-624 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Citations (Scopus)

Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells

Motohashi, T., Watanabe, N., Nishioka, M., Nakatake, Y., Yulan, P., Mochizuki, H., Kawamura, Y., Ko, M. S. H., Goshima, N. & Kunisada, T., 2016 Mar 15, In: Biology Open. 5, 3, p. 311-322 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

10 Citations (Scopus)

Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines

Yamamizu, K., Sharov, A. A., Piao, Y., Amano, M., Yu, H., Nishiyama, A., Dudekula, D. B., Schlessinger, D. & Ko, M. S. H., 2016 May 6, In: Scientific reports. 6, 25667.

Research output: Contribution to journal › Article › peer-review

Open Access

19 Citations (Scopus)

Induction of specific neuron types by overexpression of single transcription factors

Teratani-Ota, Y., Yamamizu, K., Piao, Y., Sharova, L., Amano, M., Yu, H., Schlessinger, D., Ko, M. S. H. & Sharov, A. A., 2016 Oct 1, In: In Vitro Cellular and Developmental Biology - Animal. 52, 9, p. 961-973 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

13 Citations (Scopus)

Osteoprotegerin regulates pancreatic β-cell homeostasis upon microbial invasion

Kuroda, Y., Maruyama, K., Fujii, H., Sugawara, I., Ko, S. B. H., Yasuda, H., Matsui, H. & Matsuo, K., 2016 Jan 11, In: PloS one. 11, 1, e0146544.

Research output: Contribution to journal › Article › peer-review

Open Access

16 Citations (Scopus)

Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells

Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., Ko, S. B. H. & Ko, M. S. H., 2016 Oct 15, In: Development (Cambridge). 143, 20, p. 3674-3685 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

40 Citations (Scopus)

Zygotic Genome Activation Revisited: Looking Through the Expression and Function of Zscan4

Ko, M. S. H., 2016, Mammalian Preimplantation Development, 2016. De Pamphilis, M. L. (ed.). Academic Press Inc., p. 103-124 22 p. (Current Topics in Developmental Biology; vol. 120).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

57 Citations (Scopus)

2015

ExAtlas: An interactive online tool for meta-analysis of gene expression data

Sharov, A. A., Schlessinger, D. & Ko, M. S. H., 2015 Dec 1, In: Journal of Bioinformatics and Computational Biology. 13, 6, 1550019.

Research output: Contribution to journal › Article › peer-review

Open Access

55 Citations (Scopus)

Functional characteristics of L1156F-CFTR associated with alcoholic chronic pancreatitis in Japanese

Kondo, S., Fujiki, K., Ko, S. B. H., Yamamoto, A., Nakakuki, M., Ito, Y., Shcheynikov, N., Kitagawa, M., Naruse, S. & Ishiguro, H., 2015 Aug 18, In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 309, 4, p. G260-G269

Research output: Contribution to journal › Article › peer-review

Open Access

7 Citations (Scopus)

Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells

Akiyama, T., Xin, L., Oda, M., Sharov, A. A., Amano, M., Piao, Y., Cadet, J. S., Dudekula, D. B., Qian, Y., Wang, W., Ko, S. B. H. & Ko, M. S. H., 2015 May 6, In: DNA Research. 22, 5, p. 307-318 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

72 Citations (Scopus)

2014

Amendment of the Japanese Consensus Guidelines for Autoimmune Pancreatitis, 2013 I. Concept and diagnosis of autoimmune pancreatitis

Okazaki, K., Kawa, S., Kamisawa, T., Ito, T., Inui, K., Irie, H., Nishino, T., Notohara, K., Nishimori, I., Tanaka, S., Nishiyama, T., Suda, K., Shiratori, K., Tanaka, M., Shimosegawa, T., Kubo, K., Ohara, H., Irisawa, A., Fujinaga, Y. & Hasebe, O. & 19 others, Yamamoto, H., Uchida, K., Kanno, A., Kubota, K., Ko, S., Sakagami, J., Shimizu, K., Sugiyama, M., Tada, M., Nakazawa, T., Nishino, H., Hamano, H., Hirooka, Y., Hirano, K., Masamune, A., Masuda, A., Mizuno, N., Yamaguchi, K. & Yoshida, H., 2014 Apr, In: Journal of gastroenterology. 49, 4, p. 567-588 22 p.

Research output: Contribution to journal › Article › peer-review

89 Citations (Scopus)

Unprecedented cell-selection using ultra-quick freezing combined with aquaporin expression

Kato, Y., Miyauchi, T., Abe, Y., Kojić, D., Tanaka, M., Chikazawa, N., Nakatake, Y., Ko, S. B. H., Kobayashi, D., Hazama, A., Fujiwara, S., Uchida, T. & Yasui, M., 2014 Feb 18, In: PloS one. 9, 2, e87644.

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

2013

Identification of transcription factors for lineage-specific ESC differentiation

Yamamizu, K., Piao, Y., Sharov, A. A., Zsiros, V., Yu, H., Nakazawa, K., Schlessinger, D. & Ko, M. S. H., 2013 Dec 17, In: Stem cell reports. 1, 6, p. 545-559 15 p.

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Role of iPSC-Producing Factors in Pre-Implantation Embryos

Amano, T. & Ko, M. S. H., 2013 Oct, Principles of Cloning: Second Edition. Elsevier Inc., p. 473-484 12 p.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

Morgani, S. M., Canham, M. A., Nichols, J., Sharov, A. A., Migueles, R. P., Ko, M. S. H. & Brickman, J. M., 2013 Mar 27, In: Cell Reports. 3, 6, p. 1945-1957 13 p.

Research output: Contribution to journal › Article › peer-review

188 Citations (Scopus)

2012

Deletion of Slc26a6 alters the stoichiometry of apical Cl^-/HCO₃^- exchange in mouse pancreatic duct

Ishiguro, H., Song, Y., Yamamoto, A., Steward, M. C., Ko, S. B. H., Stewart, A. K., Soleimani, M., Liu, B. C., Kondo, T. & Jin, C. X., 2012 Oct 15, In: American Journal of Physiology - Cell Physiology. 303, 8, p. C815-C824

Research output: Contribution to journal › Article › peer-review

Open Access

16 Citations (Scopus)

2011

How fast can pancreatic cancer grow? A case of pancreatic carcinoma developed within 5 months after a negative examination to the advanced stage with multiple liver and bone metastases

Ko, S. B. H. & Goto, H., 2011 Mar 1, In: Pancreas. 40, 2, p. 318-319 2 p.

Research output: Contribution to journal › Letter › peer-review

1 Citation (Scopus)

2008

Observation of deworming process in intestinal Diphyllobothrium latum parasitism by Gastrografin injection into jejunum through double-balloon enteroscope

Ko, S. B. H., Ohmiya, N., Nakamura, M., Honda, W., Shirai, O., Takenaka, H., Niwa, Y. & Goto, H., 2008 Aug, In: American Journal of Gastroenterology. 103, 8, p. 2149-2150 2 p.

Research output: Contribution to journal › Letter › peer-review

3 Citations (Scopus)

2006

Enhancement of antibacterial activity of β-lactam antibiotics by [P₂W₁₈O₆₂]^6-, [SiMo₁₂O₄₀]^4-, and [PTi₂W₁₀O₄₀]^7- against methicillin-resistant and vancomycin-resistant Staphylococcus aureus

Inoue, M., Suzuki, T., Fujita, Y., Oda, M., Matsumoto, N. & Yamase, T., 2006 Jul, In: Journal of Inorganic Biochemistry. 100, 7, p. 1225-1233 9 p.

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

2002

Differentiation of trophoblast lineage is associated with DNA methylation and demethylation

Ohgane, J., Hattori, N., Oda, M., Tanaka, S. & Shiota, K., 2002 Jan 18, In: Biochemical and Biophysical Research Communications. 290, 2, p. 701-706 6 p.

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

1989

A Highly Inducible System of Gene Expression by Positive Feedback Production of Glucocorticoid Receptors

Ko, M. S. H. & Takano, T., 1989 Mar, In: DNA. 8, 2, p. 127-133 7 p.

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

1985

Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Noda, M., Ko, M., Ogura, A., Liu, D. G., Amano, T., Takano, T. & Ikawa, Y., 1985, In: Nature. 318, 6041, p. 73-75 3 p.

Research output: Contribution to journal › Article › peer-review

408 Citations (Scopus)

The Sakaguchi Laboratory of Developmental Biology Department of Systems Medicine

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