α-Synuclein BAC transgenic mice as a model for Parkinson's disease manifested decreased anxiety-like behavior and hyperlocomotion

Hodaka Yamakado; Yasuhiro Moriwaki; Nobuyuki Yamasaki; Tsuyoshi Miyakawa; Junko Kurisu; Kengo Uemura; Haruhisa Inoue; Makio Takahashi; Ryosuke Takahashi

doi:10.1016/j.neures.2012.03.010

α-Synuclein BAC transgenic mice as a model for Parkinson's disease manifested decreased anxiety-like behavior and hyperlocomotion

Hodaka Yamakado, Yasuhiro Moriwaki, Nobuyuki Yamasaki, Tsuyoshi Miyakawa, Junko Kurisu, Kengo Uemura, Haruhisa Inoue, Makio Takahashi, Ryosuke Takahashi

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

α-Synuclein (α-syn), the main component of Lewy bodies, was identified as a genetic risk factor for idiopathic Parkinson's disease (PD). As a model for PD, we generated human α-syn bacterial artificial chromosome transgenic mice (BAC tg mice) harboring the entire human α-syn gene and its gene expression regulatory regions. The α-syn BAC tg mice manifested decreased anxiety-like behaviors which may reflect non-motor symptoms of early PD, and they exhibited increased SERT expression that may be responsible for decreased anxiety-like behaviors. Our α-syn BAC tg mice could be a valuable tool to evaluate α-syn gene dosage effects in vivo.

Original language	English
Pages (from-to)	173-177
Number of pages	5
Journal	Neuroscience Research
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/j.neures.2012.03.010
Publication status	Published - 2012 Jun

Keywords

BAC transgenic mouse
Decreased anxiety
PARK4
Parkinson's disease
Serotonin transporter
α-Synuclein

ASJC Scopus subject areas

Neuroscience(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.neures.2012.03.010

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title = "α-Synuclein BAC transgenic mice as a model for Parkinson's disease manifested decreased anxiety-like behavior and hyperlocomotion",

abstract = "α-Synuclein (α-syn), the main component of Lewy bodies, was identified as a genetic risk factor for idiopathic Parkinson's disease (PD). As a model for PD, we generated human α-syn bacterial artificial chromosome transgenic mice (BAC tg mice) harboring the entire human α-syn gene and its gene expression regulatory regions. The α-syn BAC tg mice manifested decreased anxiety-like behaviors which may reflect non-motor symptoms of early PD, and they exhibited increased SERT expression that may be responsible for decreased anxiety-like behaviors. Our α-syn BAC tg mice could be a valuable tool to evaluate α-syn gene dosage effects in vivo.",

keywords = "BAC transgenic mouse, Decreased anxiety, PARK4, Parkinson's disease, Serotonin transporter, α-Synuclein",

author = "Hodaka Yamakado and Yasuhiro Moriwaki and Nobuyuki Yamasaki and Tsuyoshi Miyakawa and Junko Kurisu and Kengo Uemura and Haruhisa Inoue and Makio Takahashi and Ryosuke Takahashi",

note = "Funding Information: We thank Dr. Sigeo Kitayama (Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan) for the gift of SERT antibody, Ms Ai Tanigaki and Ms Rie Hikawa for the technical support in immunoblotting of DAT and SERT in mice brains, Ms Yoshiko Karatsu for animal care and Dr. Roberto Gavinio in proofreading the manuscript. This study is supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 18390255 ) and is also supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan. ",

year = "2012",

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doi = "10.1016/j.neures.2012.03.010",

language = "English",

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AU - Yamakado, Hodaka

AU - Moriwaki, Yasuhiro

AU - Yamasaki, Nobuyuki

AU - Miyakawa, Tsuyoshi

AU - Kurisu, Junko

AU - Uemura, Kengo

AU - Inoue, Haruhisa

AU - Takahashi, Makio

AU - Takahashi, Ryosuke

N1 - Funding Information: We thank Dr. Sigeo Kitayama (Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan) for the gift of SERT antibody, Ms Ai Tanigaki and Ms Rie Hikawa for the technical support in immunoblotting of DAT and SERT in mice brains, Ms Yoshiko Karatsu for animal care and Dr. Roberto Gavinio in proofreading the manuscript. This study is supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 18390255 ) and is also supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2012/6

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N2 - α-Synuclein (α-syn), the main component of Lewy bodies, was identified as a genetic risk factor for idiopathic Parkinson's disease (PD). As a model for PD, we generated human α-syn bacterial artificial chromosome transgenic mice (BAC tg mice) harboring the entire human α-syn gene and its gene expression regulatory regions. The α-syn BAC tg mice manifested decreased anxiety-like behaviors which may reflect non-motor symptoms of early PD, and they exhibited increased SERT expression that may be responsible for decreased anxiety-like behaviors. Our α-syn BAC tg mice could be a valuable tool to evaluate α-syn gene dosage effects in vivo.

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KW - Serotonin transporter

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α-Synuclein BAC transgenic mice as a model for Parkinson's disease manifested decreased anxiety-like behavior and hyperlocomotion

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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