TY - JOUR
T1 - β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity
AU - Park, Hyun Young
AU - Toume, Kazufumi
AU - Arai, Midori A.
AU - Koyano, Takashi
AU - Kowithayakorn, Thaworn
AU - Ishibashi, Masami
N1 - Funding Information:
Acknowledgments We are grateful to Prof. J. Nathans (Johns Hopkins University School of Medicine) for the STF/293 cells and Prof. R. T. Moon (University of Washington) for the SuperTOPFlash plasmid. This study was supported by Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the Cosmetology Research Foundation, and Sekisui Chemical Innovations Inspired by Nature Research Support Program.
PY - 2014/1
Y1 - 2014/1
N2 - Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.
AB - Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.
KW - Bauhinia malabarica
KW - Flavonoid
KW - Wnt signaling pathway
KW - β-Catenin
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U2 - 10.1007/s11418-013-0762-3
DO - 10.1007/s11418-013-0762-3
M3 - Article
C2 - 23516045
AN - SCOPUS:84893728174
SN - 1340-3443
VL - 68
SP - 242
EP - 245
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 1
ER -