1000 spider silkomes: Linking sequences to silk physical properties

Kazuharu Arakawa; Nobuaki Kono; Ali D. Malay; Ayaka Tateishi; Nao Ifuku; Hiroyasu Masunaga; Ryota Sato; Kousuke Tsuchiya; Rintaro Ohtoshi; Daniel Pedrazzoli; Asaka Shinohara; Yusuke Ito; Hiroyuki Nakamura; Akio Tanikawa; Yuya Suzuki; Takeaki Ichikawa; Shohei Fujita; Masayuki Fujiwara; Masaru Tomita; Sean J. Blamires; Jo Ann Chuah; Hamish Craig; Choon P. Foong; Gabriele Greco; Juan Guan; Chris Holland; David L. Kaplan; Kumar Sudesh; Biman B. Mandal; Y. Norma-Rashid; Nur A. Oktaviani; Rucsanda C. Preda; Nicola M. Pugno; Rangam Rajkhowa; Xiaoqin Wang; Kenjiro Yazawa; Zhaozhu Zheng; Keiji Numata

doi:10.1126/sciadv.abo6043

1000 spider silkomes: Linking sequences to silk physical properties

Kazuharu Arakawa, Nobuaki Kono, Ali D. Malay, Ayaka Tateishi, Nao Ifuku, Hiroyasu Masunaga, Ryota Sato, Kousuke Tsuchiya, Rintaro Ohtoshi, Daniel Pedrazzoli, Asaka Shinohara, Yusuke Ito, Hiroyuki Nakamura, Akio Tanikawa, Yuya Suzuki, Takeaki Ichikawa, Shohei Fujita, Masayuki Fujiwara, Masaru Tomita, Sean J. BlamiresJo Ann Chuah, Hamish Craig, Choon P. Foong, Gabriele Greco, Juan Guan, Chris Holland, David L. Kaplan, Kumar Sudesh, Biman B. Mandal, Y. Norma-Rashid, Nur A. Oktaviani, Rucsanda C. Preda, Nicola M. Pugno, Rangam Rajkhowa, Xiaoqin Wang, Kenjiro Yazawa, Zhaozhu Zheng, Keiji Numata

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23 Citations (Scopus)

Abstract

Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.

Original language	English
Journal	Science Advances
Volume	8
Issue number	41
DOIs	https://doi.org/10.1126/sciadv.abo6043
Publication status	Published - 2022 Oct

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Arakawa, K., Kono, N., Malay, A. D., Tateishi, A., Ifuku, N., Masunaga, H., Sato, R., Tsuchiya, K., Ohtoshi, R., Pedrazzoli, D., Shinohara, A., Ito, Y., Nakamura, H., Tanikawa, A., Suzuki, Y., Ichikawa, T., Fujita, S., Fujiwara, M., Tomita, M., ... Numata, K. (2022). 1000 spider silkomes: Linking sequences to silk physical properties. Science Advances, 8(41). https://doi.org/10.1126/sciadv.abo6043

Arakawa, K , Kono, N, Malay, AD, Tateishi, A, Ifuku, N, Masunaga, H, Sato, R, Tsuchiya, K, Ohtoshi, R, Pedrazzoli, D, Shinohara, A, Ito, Y, Nakamura, H, Tanikawa, A, Suzuki, Y, Ichikawa, T, Fujita, S, Fujiwara, M, Tomita, M, Blamires, SJ, Chuah, JA, Craig, H, Foong, CP, Greco, G, Guan, J, Holland, C, Kaplan, DL, Sudesh, K, Mandal, BB, Norma-Rashid, Y, Oktaviani, NA, Preda, RC, Pugno, NM, Rajkhowa, R, Wang, X, Yazawa, K, Zheng, Z & Numata, K 2022, '1000 spider silkomes: Linking sequences to silk physical properties', Science Advances, vol. 8, no. 41. https://doi.org/10.1126/sciadv.abo6043

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title = "1000 spider silkomes: Linking sequences to silk physical properties",

abstract = "Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.",

author = "Kazuharu Arakawa and Nobuaki Kono and Malay, {Ali D.} and Ayaka Tateishi and Nao Ifuku and Hiroyasu Masunaga and Ryota Sato and Kousuke Tsuchiya and Rintaro Ohtoshi and Daniel Pedrazzoli and Asaka Shinohara and Yusuke Ito and Hiroyuki Nakamura and Akio Tanikawa and Yuya Suzuki and Takeaki Ichikawa and Shohei Fujita and Masayuki Fujiwara and Masaru Tomita and Blamires, {Sean J.} and Chuah, {Jo Ann} and Hamish Craig and Foong, {Choon P.} and Gabriele Greco and Juan Guan and Chris Holland and Kaplan, {David L.} and Kumar Sudesh and Mandal, {Biman B.} and Y. Norma-Rashid and Oktaviani, {Nur A.} and Preda, {Rucsanda C.} and Pugno, {Nicola M.} and Rangam Rajkhowa and Xiaoqin Wang and Kenjiro Yazawa and Zhaozhu Zheng and Keiji Numata",

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AU - Arakawa, Kazuharu

AU - Kono, Nobuaki

AU - Malay, Ali D.

AU - Tateishi, Ayaka

AU - Ifuku, Nao

AU - Masunaga, Hiroyasu

AU - Sato, Ryota

AU - Tsuchiya, Kousuke

AU - Ohtoshi, Rintaro

AU - Pedrazzoli, Daniel

AU - Shinohara, Asaka

AU - Ito, Yusuke

AU - Nakamura, Hiroyuki

AU - Tanikawa, Akio

AU - Suzuki, Yuya

AU - Ichikawa, Takeaki

AU - Fujita, Shohei

AU - Fujiwara, Masayuki

AU - Tomita, Masaru

AU - Blamires, Sean J.

AU - Chuah, Jo Ann

AU - Craig, Hamish

AU - Foong, Choon P.

AU - Greco, Gabriele

AU - Guan, Juan

AU - Holland, Chris

AU - Kaplan, David L.

AU - Sudesh, Kumar

AU - Mandal, Biman B.

AU - Norma-Rashid, Y.

AU - Oktaviani, Nur A.

AU - Preda, Rucsanda C.

AU - Pugno, Nicola M.

AU - Rajkhowa, Rangam

AU - Wang, Xiaoqin

AU - Yazawa, Kenjiro

AU - Zheng, Zhaozhu

AU - Numata, Keiji

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1000 spider silkomes: Linking sequences to silk physical properties

Abstract

ASJC Scopus subject areas

UN SDGs

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