3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

Kenichi Horisawa; Takao Imai; Hideyuki Okano; Hiroshi Yanagawa

doi:10.1016/j.febslet.2009.06.045

3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

Kenichi Horisawa, Takao Imai, Hideyuki Okano, Hiroshi Yanagawa

Department of Physiology

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.

Original language	English
Pages (from-to)	2429-2434
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	14
DOIs	https://doi.org/10.1016/j.febslet.2009.06.045
Publication status	Published - 2009 Jul 21

Keywords

3′-Untranslated region
Doublecortin
Musashi1
Neural stem cell
RNA-binding protein
Translational repression

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.06.045

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title = "3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein",

abstract = "Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.",

keywords = "3′-Untranslated region, Doublecortin, Musashi1, Neural stem cell, RNA-binding protein, Translational repression",

author = "Kenichi Horisawa and Takao Imai and Hideyuki Okano and Hiroshi Yanagawa",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for the Keio University Global-COE Program entitled {\textquoteleft}Center of Human Metabolomic Systems Biology{\textquoteright} and by a Grant-in-Aid for H.O. from the MEXT, Japan, and a grant from Takeda Science Foundation. ",

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doi = "10.1016/j.febslet.2009.06.045",

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T1 - 3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

AU - Horisawa, Kenichi

AU - Imai, Takao

AU - Okano, Hideyuki

AU - Yanagawa, Hiroshi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for the Keio University Global-COE Program entitled ‘Center of Human Metabolomic Systems Biology’ and by a Grant-in-Aid for H.O. from the MEXT, Japan, and a grant from Takeda Science Foundation.

PY - 2009/7/21

Y1 - 2009/7/21

N2 - Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.

AB - Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.

KW - 3′-Untranslated region

KW - Doublecortin

KW - Musashi1

KW - Neural stem cell

KW - RNA-binding protein

KW - Translational repression

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JO - FEBS Letters

JF - FEBS Letters

IS - 14

ER -

3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

Abstract

Keywords

ASJC Scopus subject areas

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