3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo

H. Watanabe, Y. Ishihara, K. Okamoto, K. Oshio, T. Kanamatsu, Y. Tsukada

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1J(CH)) before the 90°(1H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°(1H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1H-13C correlation spectra from a monkey brain in vivo were obtained after [1-13C]glucose injection, and amino acid metabolism was detected; that is, [4-13C]glutamate appeared immediately after the injection, followed by the appearance of [2- 13C]glutamate, [3-13C]glutamate, and [4-13C]glutamine. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish
Pages (from-to)200-210
Number of pages11
JournalMagnetic Resonance in Medicine
Issue number2
Publication statusPublished - 2000
Externally publishedYes


  • Amino acid metabolism
  • H-C HSQC correlation
  • In vivo
  • MRS
  • Spatial localization

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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