3D visualization of epidermal langerhans cells

Akiharu Kubo, Keisuke Nagao, Masayuki Amagai

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)


Langerhans cells (LCs) are intraepidermal dendritic cells that extend their dendrites between keratinocytes to form a dense network that covers the entire body. The mammalian epidermis has two diffusion barriers, the stratum corneum and tight junctions (TJs). In their resting state, LCs sit underneath these two barriers. Once activated, LCs elongate their dendrites to dock with, or penetrate through, TJs, which allows them to survey the environment between the two barriers. Here, we describe a method to visualize this dynamic interaction of TJs and LCs in 3D by using mouse ear epidermal sheets.

Original languageEnglish
Title of host publicationMolecular Dermatology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages9
ISBN (Print)9781627032261
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Langerhans cells
  • Skin-resident dendritic cells
  • Stratum corneum
  • Tight junction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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