A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

Masanori Sakaguchi; Tetsuro Shingo; Takuya Shimazaki; Hirotaka James Okano; Mieko Shiwa; Satoru Ishibashi; Hideyuki Oguro; Mikiko Ninomiya; Toshihiko Kadoya; Hidenori Horie; Akira Shibuya; Hidehiro Mizusawa; Françoise Poirier; Hiromitsu Nakauchi; Kazunobu Sawamoto; Hideyuki Okano

doi:10.1073/pnas.0508793103

A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

Masanori Sakaguchi, Tetsuro Shingo, Takuya Shimazaki, Hirotaka James Okano, Mieko Shiwa, Satoru Ishibashi, Hideyuki Oguro, Mikiko Ninomiya, Toshihiko Kadoya, Hidenori Horie, Akira Shibuya, Hidehiro Mizusawa, Françoise Poirier, Hiromitsu Nakauchi, Kazunobu Sawamoto, Hideyuki Okano

Department of Physiology

Research output: Contribution to journal › Article › peer-review

146 Citations (Scopus)

Abstract

In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.

Original language	English
Pages (from-to)	7112-7117
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	18
DOIs	https://doi.org/10.1073/pnas.0508793103
Publication status	Published - 2006 May 2

Keywords

Lectin
Mobilization
Stem cell niche

ASJC Scopus subject areas

General

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10.1073/pnas.0508793103

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Sakaguchi, M., Shingo, T., Shimazaki, T., Okano, H. J., Shiwa, M., Ishibashi, S., Oguro, H., Ninomiya, M., Kadoya, T., Horie, H., Shibuya, A., Mizusawa, H., Poirier, F., Nakauchi, H., Sawamoto, K., & Okano, H. (2006). A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. Proceedings of the National Academy of Sciences of the United States of America, 103(18), 7112-7117. https://doi.org/10.1073/pnas.0508793103

Sakaguchi, M, Shingo, T, Shimazaki, T, Okano, HJ, Shiwa, M, Ishibashi, S, Oguro, H, Ninomiya, M, Kadoya, T, Horie, H, Shibuya, A, Mizusawa, H, Poirier, F, Nakauchi, H, Sawamoto, K & Okano, H 2006, 'A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 18, pp. 7112-7117. https://doi.org/10.1073/pnas.0508793103

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abstract = "In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.",

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AU - Okano, Hirotaka James

AU - Shiwa, Mieko

AU - Ishibashi, Satoru

AU - Oguro, Hideyuki

AU - Ninomiya, Mikiko

AU - Kadoya, Toshihiko

AU - Horie, Hidenori

AU - Shibuya, Akira

AU - Mizusawa, Hidehiro

AU - Poirier, Françoise

AU - Nakauchi, Hiromitsu

AU - Sawamoto, Kazunobu

AU - Okano, Hideyuki

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AB - In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.

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KW - Mobilization

KW - Stem cell niche

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A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells

Abstract

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