TY - GEN
T1 - A high-throughput drug-screening system targeting ABC-transporters
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
AU - Abe, Y.
AU - Sasaki, H.
AU - Yamamoto, R.
AU - Osaki, T.
AU - Kawano, R.
AU - Kamiya, K.
AU - Miki, N.
AU - Takeuchi, S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.
AB - We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.
KW - ABC-transporter
KW - Concentration gradient
KW - Microfluidics
KW - PDMS
UR - http://www.scopus.com/inward/record.url?scp=84883776778&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883776778&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883776778
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 885
EP - 887
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -