@inbook{af4629d476f34e1db08df3c228749764,
title = "A Method for Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells",
abstract = "Human induced pluripotent stem cells (hiPSCs) are one of the most promising cell sources for regenerative medicine. To realize the promise of hiPSCs for cardiac regenerative therapy, three major obstacles must be overcome: the first is the achievement of large-scale production of cardiomyocytes, the second is the successful elimination of non-cardiac cells containing residual pluripotent stem cells (PSCs) to prevent tumor formation, and the third is the achievement of high engraftment efficiency of transplanted cardiomyocytes. In this chapter, we introduce our protocols for cardiac differentiation, purification, and preparation of cardiac spheroids for safe and effective regenerative medicine.",
keywords = "Cardiomyocytes, Differentiation, Induced pluripotent stem cells (iPSCs), Purification, Spheroid, Transplantation",
author = "Yuika Morita and Shugo Tohyama and Jun Fujita and Keiichi Fukuda",
note = "Funding Information: The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development grant (19bm0404023h0002 to S. T.), JSPS KAKENHI grant (20J01097 to Y.M.) and JSPS KAKENHI grant (19K22626 to S. T.). Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1484-6_2",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "11--21",
booktitle = "Methods in Molecular Biology",
}
