TY - GEN
T1 - A microfluidic organoid trapping device to form tube-like intestinal organoids
AU - Matsumoto, Miki
AU - Morimoto, Yuya
AU - Sato, Toshiro
AU - Takeuchi, Shoji
N1 - Funding Information:
We thank K. Arai from JSR corporation for his technical support. This work was partially supported by JST-Mirai Program JPMJMI18CE, JSPS Grants-in-Aid for Scientific Research 16H06329 and 18K18991, and Organoid Project at RIKEN Center for Biosystems Dynamics Research.
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - We propose an organoid-trapping device to form long intestinal epithelial organoids. The device is drivable with hydrostatic pressure to gather standard organoids, and induces their fusion in the microchannel. We can control the length of the tube-like organoids by adjusting the number of standard organoids. As a demonstration, six organoids were trapped in the device and tube-like organoids were formed by their fusion within two days. Our device will provide desired length of organoids for development of a perfusable 3D intestinal organoid model.
AB - We propose an organoid-trapping device to form long intestinal epithelial organoids. The device is drivable with hydrostatic pressure to gather standard organoids, and induces their fusion in the microchannel. We can control the length of the tube-like organoids by adjusting the number of standard organoids. As a demonstration, six organoids were trapped in the device and tube-like organoids were formed by their fusion within two days. Our device will provide desired length of organoids for development of a perfusable 3D intestinal organoid model.
KW - Fusion
KW - Intestinal organoid
KW - Microfluidics
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M3 - Conference contribution
AN - SCOPUS:85098260226
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 871
EP - 872
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -