A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering

Satoru Tokutomi; Masayoshi Nakasako; Jun Sakai; Mikio Kataoka; Kotaro T. Yamamoto; Masamitsu Wada; Fumio Tokunaga; Masaki Furuya

doi:10.1016/0014-5793(89)81257-8

A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering

Satoru Tokutomi, Masayoshi Nakasako, Jun Sakai, Mikio Kataoka, Kotaro T. Yamamoto, Masamitsu Wada, Fumio Tokunaga, Masaki Furuya

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a 'four-leaved shape' model is proposed. The propriety of the model was confirmed by images of the molecule obtained by rotary shadowing electron microscopy.

Original language	English
Pages (from-to)	139-142
Number of pages	4
Journal	FEBS Letters
Volume	247
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81257-8
Publication status	Published - 1989 Apr 10
Externally published	Yes

Keywords

Domain structure
Molecular structure
Phytochrome
Small-angle X-ray scattering

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)81257-8

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title = "A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering",

abstract = "The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a 'four-leaved shape' model is proposed. The propriety of the model was confirmed by images of the molecule obtained by rotary shadowing electron microscopy.",

keywords = "Domain structure, Molecular structure, Phytochrome, Small-angle X-ray scattering",

author = "Satoru Tokutomi and Masayoshi Nakasako and Jun Sakai and Mikio Kataoka and Yamamoto, {Kotaro T.} and Masamitsu Wada and Fumio Tokunaga and Masaki Furuya",

note = "Funding Information: AcknowledgementWs:e expresso ur sinceret hankst o Professor Tatzuo Ueki and the other memberso f the SAXES working group for their technicala dvice,t o Professor Mitsuo Tasumi for encouragingo ur study, and to MS Yukiko Tsuge and Nobuko Naito for technicala ssistanceT. his work was supported in part by Grants-in-Aid from the Ministry of Education, Sciencea ndC ulture,a ndw asp erformedw ith thea pproval of the Photon Factory Program Advisory Committee.",

year = "1989",

month = apr,

day = "10",

doi = "10.1016/0014-5793(89)81257-8",

language = "English",

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T1 - A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering

AU - Tokutomi, Satoru

AU - Nakasako, Masayoshi

AU - Sakai, Jun

AU - Kataoka, Mikio

AU - Yamamoto, Kotaro T.

AU - Wada, Masamitsu

AU - Tokunaga, Fumio

AU - Furuya, Masaki

N1 - Funding Information: AcknowledgementWs:e expresso ur sinceret hankst o Professor Tatzuo Ueki and the other memberso f the SAXES working group for their technicala dvice,t o Professor Mitsuo Tasumi for encouragingo ur study, and to MS Yukiko Tsuge and Nobuko Naito for technicala ssistanceT. his work was supported in part by Grants-in-Aid from the Ministry of Education, Sciencea ndC ulture,a ndw asp erformedw ith thea pproval of the Photon Factory Program Advisory Committee.

PY - 1989/4/10

Y1 - 1989/4/10

N2 - The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a 'four-leaved shape' model is proposed. The propriety of the model was confirmed by images of the molecule obtained by rotary shadowing electron microscopy.

AB - The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a 'four-leaved shape' model is proposed. The propriety of the model was confirmed by images of the molecule obtained by rotary shadowing electron microscopy.

KW - Domain structure

KW - Molecular structure

KW - Phytochrome

KW - Small-angle X-ray scattering

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AN - SCOPUS:0001577686

SN - 0014-5793

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SP - 139

EP - 142

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

A model for the dimeric molecular structure of phytochrome based on small-angle X-ray scattering

Abstract

Keywords

ASJC Scopus subject areas

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