A Nanopore Sequencing Course for Graduate School Curriculum

Research output: Contribution to journalArticlepeer-review


The high-throughput long-read sequencing has become affordable enough for any molecular biology lab to utilize genome sequencing in their research. Complete genome sequencing and assembly of bacterial genomes is one such application which is powerful yet simple enough for anyone without advanced molecular biology or bioinformatics skills to conduct on his/her own. High-throughput sequencing will eventually become a basic routine tool in molecular biology labs just like polymerase chain reaction and electrophoresis in a near future. To assist the use of such nanopore sequencing technologies, we designed a graduate school course to learn both the experimental and bioinformatic skills of complete bacterial genome sequencing and assembly.

Original languageEnglish
Pages (from-to)113-127
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2023


  • Complete bacterial genome sequencing
  • De novo assembly
  • Education
  • Genome annotation
  • Nanopore sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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