A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies

Kenta Noda, Kouki Matsuda, Shigehiro Yagishita, Kenji Maeda, Yutaro Akiyama, Junko Terada-Hirashima, Hiromichi Matsushita, Satoshi Iwata, Kazuto Yamashita, Yusuke Atarashi, Shunsuke Watanabe, Nobuyuki Ide, Tomokazu Yoshida, Norio Ohmagari, Hiroaki Mitsuya, Akinobu Hamada

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


The quantitative range and reproducibility of current serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are not optimized. Herein, we developed a diagnostic test that detects SARS-CoV-2 IgG and IgM with high quantitativeness and reproducibility and low interference. The system was based on the high-sensitivity chemiluminescence enzyme immunoassay (HISCL) platform and detects IgG and IgM specific to SARS-CoV-2 spike and nucleocapsid proteins. Quantification accuracy and reproducibility were evaluated using serially diluted samples from 60 SARS-CoV-2-infected patients. Assay performance was evaluated using serum samples from the SARS-CoV-2-infected patients and 500 SARS-CoV-2-negative serum samples collected before the emergence of SARS-CoV-2. The system showed high quantification accuracy (range, 102), high reproducibility (within 5%), and no cross-reaction between SARS1- and MERS-S proteins. Detection accuracy was 98.3% and 93.3% for IgG and IgM against spike proteins and 100% and 71.7% for IgG and IgM against nucleocapsid proteins, respectively. Mean antibody levels were > 10 times that in negative samples upon admission and > 100 times that at convalescent periods. Clinical severity upon admission was not correlated with IgG or IgM levels. This highly quantitative, reproducible assay system with high clinical performance may help analyze temporal serological/immunological profiles of SARS-CoV-2 infection and SARS-CoV-2 vaccine effectiveness.

Original languageEnglish
Article number5198
JournalScientific reports
Issue number1
Publication statusPublished - 2021 Dec
Externally publishedYes

ASJC Scopus subject areas

  • General


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