A novel Xenopus laevis SRY-related gene, xSox33

Jun Hagiuda; Yoshiki Hiraoka; Masanori Hasegawa; Motoyuki Ogawa; Sadakazu Aiso

doi:10.1016/S0167-4781(03)00139-8

A novel Xenopus laevis SRY-related gene, xSox33

Jun Hagiuda, Yoshiki Hiraoka, Masanori Hasegawa, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

Original language	English
Pages (from-to)	140-145
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1628
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(03)00139-8
Publication status	Published - 2003 Jul 28
Externally published	Yes

Keywords

(Xenopus laevis)
DNA binding
Embryogenesis
HMG box
SOX
SRY

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(03)00139-8

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title = "A novel Xenopus laevis SRY-related gene, xSox33",

abstract = "The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.",

keywords = "(Xenopus laevis), DNA binding, Embryogenesis, HMG box, SOX, SRY",

author = "Jun Hagiuda and Yoshiki Hiraoka and Masanori Hasegawa and Motoyuki Ogawa and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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AU - Hiraoka, Yoshiki

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AU - Ogawa, Motoyuki

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

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Y1 - 2003/7/28

N2 - The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

AB - The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

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KW - HMG box

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A novel Xenopus laevis SRY-related gene, xSox33

Abstract

Keywords

ASJC Scopus subject areas

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