TY - JOUR
T1 - A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies
AU - Obata, Ken'ichi
AU - Iwata, Kazushi
AU - Okada, Yasunori
AU - Kohrin, Yumi
AU - Ohuchi, Eiko
AU - Yoshida, Shin'ichi
AU - Shinmei, Masayuki
AU - Hayakawa, Taro
PY - 1992/10/15
Y1 - 1992/10/15
N2 - A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.
AB - A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.
KW - Monoclonal antibody
KW - Osteoarthritis
KW - Rheumatoid arthritis
KW - Sandwich enzyme immunoassay
KW - Stromelysin-1
UR - http://www.scopus.com/inward/record.url?scp=0026453522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026453522&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(92)90105-Y
DO - 10.1016/0009-8981(92)90105-Y
M3 - Article
C2 - 1281763
AN - SCOPUS:0026453522
SN - 0009-8981
VL - 211
SP - 59
EP - 72
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -