A placenta-specific 5 ' non-coding exon of human prolactin

Hiraoka Yoshiki; Tatsumi Keita; Shiozawa Masahide; Also Sadakazu; Fukasawa Toshio; Yasuda Kenjiro; Miyai Kiyoshi

doi:10.1016/0303-7207(91)90247-P

A placenta-specific 5 ' non-coding exon of human prolactin

Hiraoka Yoshiki, Tatsumi Keita, Shiozawa Masahide, Also Sadakazu, Fukasawa Toshio, Yasuda Kenjiro, Miyai Kiyoshi

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Expression of human prolactin (hPRL) is restricted to the anterior pituitary and the placenta, strictly, the decidualized endometrium of the uterus. We have cloned and sequenced placental hPRL cDNAs and also the 5' part of the hPRL gene, and found that the placental mRNA consisted of six exons including a 5' non-coding exon which was not present in the pituitary mRNA known to consist of five exons. The placenta-specific exon, designated exon 0, was located 5.3 kb upstream of the first exon of the pituitary mRNA. We have also located the transcriptional start site of the hPRL gene in the placenta to be 21 bp upstream from the 5' end of the longest cDNA clone we obtained using mRNA from human placenta of 22 weeks of gestation. These results suggest that the hPRL gene contains a placenta-specific promoter other than the pituitary-specific promoter.

Original language	English
Pages (from-to)	71-80
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/0303-7207(91)90247-P
Publication status	Published - 1991 Jan
Externally published	Yes

Keywords

Decidua
Exon, 5' non-coding
Prolactin, human
Tissue-specific expression

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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title = "A placenta-specific 5 ' non-coding exon of human prolactin",

abstract = "Expression of human prolactin (hPRL) is restricted to the anterior pituitary and the placenta, strictly, the decidualized endometrium of the uterus. We have cloned and sequenced placental hPRL cDNAs and also the 5' part of the hPRL gene, and found that the placental mRNA consisted of six exons including a 5' non-coding exon which was not present in the pituitary mRNA known to consist of five exons. The placenta-specific exon, designated exon 0, was located 5.3 kb upstream of the first exon of the pituitary mRNA. We have also located the transcriptional start site of the hPRL gene in the placenta to be 21 bp upstream from the 5' end of the longest cDNA clone we obtained using mRNA from human placenta of 22 weeks of gestation. These results suggest that the hPRL gene contains a placenta-specific promoter other than the pituitary-specific promoter.",

keywords = "Decidua, Exon, 5' non-coding, Prolactin, human, Tissue-specific expression",

author = "Hiraoka Yoshiki and Tatsumi Keita and Shiozawa Masahide and Also Sadakazu and Fukasawa Toshio and Yasuda Kenjiro and Miyai Kiyoshi",

note = "Funding Information: We thank Drs. T. Tokino and H. Yokouchi for giving the cosmid libraries of the human genome, Dr. K. Matsubara for his generosity for K.T. to use facilities of his laboratory and Dr. H. Nari-matsu for his helpful discussion. This work was supported in part by grants from the Ministry of Education, Science and Culture to Y.H. and M.S. and from the Ministry of Agriculture, Fishery, and Forestry to T.F.",

year = "1991",

month = jan,

doi = "10.1016/0303-7207(91)90247-P",

language = "English",

volume = "75",

pages = "71--80",

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AU - Yoshiki, Hiraoka

AU - Keita, Tatsumi

AU - Masahide, Shiozawa

AU - Sadakazu, Also

AU - Toshio, Fukasawa

AU - Kenjiro, Yasuda

AU - Kiyoshi, Miyai

N1 - Funding Information: We thank Drs. T. Tokino and H. Yokouchi for giving the cosmid libraries of the human genome, Dr. K. Matsubara for his generosity for K.T. to use facilities of his laboratory and Dr. H. Nari-matsu for his helpful discussion. This work was supported in part by grants from the Ministry of Education, Science and Culture to Y.H. and M.S. and from the Ministry of Agriculture, Fishery, and Forestry to T.F.

PY - 1991/1

Y1 - 1991/1

N2 - Expression of human prolactin (hPRL) is restricted to the anterior pituitary and the placenta, strictly, the decidualized endometrium of the uterus. We have cloned and sequenced placental hPRL cDNAs and also the 5' part of the hPRL gene, and found that the placental mRNA consisted of six exons including a 5' non-coding exon which was not present in the pituitary mRNA known to consist of five exons. The placenta-specific exon, designated exon 0, was located 5.3 kb upstream of the first exon of the pituitary mRNA. We have also located the transcriptional start site of the hPRL gene in the placenta to be 21 bp upstream from the 5' end of the longest cDNA clone we obtained using mRNA from human placenta of 22 weeks of gestation. These results suggest that the hPRL gene contains a placenta-specific promoter other than the pituitary-specific promoter.

AB - Expression of human prolactin (hPRL) is restricted to the anterior pituitary and the placenta, strictly, the decidualized endometrium of the uterus. We have cloned and sequenced placental hPRL cDNAs and also the 5' part of the hPRL gene, and found that the placental mRNA consisted of six exons including a 5' non-coding exon which was not present in the pituitary mRNA known to consist of five exons. The placenta-specific exon, designated exon 0, was located 5.3 kb upstream of the first exon of the pituitary mRNA. We have also located the transcriptional start site of the hPRL gene in the placenta to be 21 bp upstream from the 5' end of the longest cDNA clone we obtained using mRNA from human placenta of 22 weeks of gestation. These results suggest that the hPRL gene contains a placenta-specific promoter other than the pituitary-specific promoter.

KW - Decidua

KW - Exon, 5' non-coding

KW - Prolactin, human

KW - Tissue-specific expression

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A placenta-specific 5 ' non-coding exon of human prolactin

Abstract

Keywords

ASJC Scopus subject areas

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