A portable lipid bilayer system for environmental sensing with a transmembrane protein

Ryuji Kawano; Yutaro Tsuji; Koki Kamiya; Taiga Kodama; Toshihisa Osaki; Norihisa Miki; Shoji Takeuchi

doi:10.1371/journal.pone.0102427

A portable lipid bilayer system for environmental sensing with a transmembrane protein

Ryuji Kawano, Yutaro Tsuji, Koki Kamiya, Taiga Kodama, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.

Original language	English
Article number	e102427
Journal	PloS one
Volume	9
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0102427
Publication status	Published - 2014 Jul 29

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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10.1371/journal.pone.0102427

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AU - Miki, Norihisa

AU - Takeuchi, Shoji

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A portable lipid bilayer system for environmental sensing with a transmembrane protein

Abstract

ASJC Scopus subject areas

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