A role for hematopoietic stem cells in promoting angiogenesis

Nobuyuki Takakura, Toshio Watanabe, Souichi Suenobu, Yoshihiro Yamada, Tetsuo Noda, Yoshiaki Ito, Masanobu Satake, Toshio Suda

Research output: Contribution to journalArticlepeer-review

483 Citations (Scopus)


Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embryos, which lack definitive hematopoiesis. These embryos showed defective angiogenesis in the head and pericardium. Para-aortic splanchnopleural (P-Sp) explant cultures on stromal cells (P-Sp culture) did not generate definitive hematopoietic cells and showed defective angiogenesis in the AML1 null embryo. Disrupted angiogenesis in P-Sp cultures from AML1 null embryos was rescued by addition of HSCs or angiopoietin-1 (Ang1). HSCs, which express Ang1, directly promoted migration of ECs in vivo and in vitro. These results indicate that HSCs are critical for angiogenesis.

Original languageEnglish
Pages (from-to)199-209
Number of pages11
Issue number2
Publication statusPublished - 2000 Jul 21
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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