TY - JOUR
T1 - A role for hematopoietic stem cells in promoting angiogenesis
AU - Takakura, Nobuyuki
AU - Watanabe, Toshio
AU - Suenobu, Souichi
AU - Yamada, Yoshihiro
AU - Noda, Tetsuo
AU - Ito, Yoshiaki
AU - Satake, Masanobu
AU - Suda, Toshio
N1 - Funding Information:
We thank Drs. Michael L. Mucenski, Masaru Okabe, and G. D. Yanchopoulos for providing us with c-Myb mutant mice, GFP transgenic mice, and Ang1 mutant mice, respectively. This work was supported in part by grants-in-aid for scientific research (06277104 and 10470211) from the Ministry of Education, Science, and Culture of Japan.
PY - 2000/7/21
Y1 - 2000/7/21
N2 - Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embryos, which lack definitive hematopoiesis. These embryos showed defective angiogenesis in the head and pericardium. Para-aortic splanchnopleural (P-Sp) explant cultures on stromal cells (P-Sp culture) did not generate definitive hematopoietic cells and showed defective angiogenesis in the AML1 null embryo. Disrupted angiogenesis in P-Sp cultures from AML1 null embryos was rescued by addition of HSCs or angiopoietin-1 (Ang1). HSCs, which express Ang1, directly promoted migration of ECs in vivo and in vitro. These results indicate that HSCs are critical for angiogenesis.
AB - Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embryos, which lack definitive hematopoiesis. These embryos showed defective angiogenesis in the head and pericardium. Para-aortic splanchnopleural (P-Sp) explant cultures on stromal cells (P-Sp culture) did not generate definitive hematopoietic cells and showed defective angiogenesis in the AML1 null embryo. Disrupted angiogenesis in P-Sp cultures from AML1 null embryos was rescued by addition of HSCs or angiopoietin-1 (Ang1). HSCs, which express Ang1, directly promoted migration of ECs in vivo and in vitro. These results indicate that HSCs are critical for angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0034697966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034697966&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)00025-8
DO - 10.1016/S0092-8674(00)00025-8
M3 - Article
C2 - 10943840
AN - SCOPUS:0034697966
SN - 0092-8674
VL - 102
SP - 199
EP - 209
JO - Cell
JF - Cell
IS - 2
ER -