Accumulated photon-echo spectroscopy for stained tissue samples

K. Uchikawa, M. Sakamoto, A. Koreeda, S. Saikan

Research output: Contribution to journalArticlepeer-review


We performed phase relaxation-time measurements of the accumulated photon echo for normal and cancerous tissue samples of human liver, that were stained with several kinds of dye. Each dye interacted selectively with a specific kind of biomolecule in the tissues, for example, DNA, proteins, or lipids. The cancerous tissues showed a tendency to have shorter relaxation times than the normal tissues had. The difference of relaxation time observed between the normal and the cancerous tissues depended on the dyes that were used for staining, and the difference became most clear when the tissues were stained with a cyanine dye derivative, i.e., YOPRo3 Iodide, which has a high selectivity for DNA. We performed the relaxation-time mapping of the accumulated photon echo (photon-echo imaging) for a liver tissue sample stained by YO-PR03 Iodide. The photonecho imaging was successful in showing a region of a highly differentiated hepatocellular carcinoma in the tissue sample, while fluorescence-intensity mapping could not identify the cancerous region in the same tissue sample. These results indicated a possibility that the reduction of accumulated photon-echo relaxation time observed in the cancerous tissues was caused by a change of microscopic dynamics of DNA.

Original languageEnglish
Pages (from-to)1058-1067
Number of pages10
JournalJournal of the Optical Society of America B: Optical Physics
Issue number6
Publication statusPublished - 2000 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Atomic and Molecular Physics, and Optics


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