TY - JOUR
T1 - Acetylcholine synthesis is modulated by acetylcholine content of cytosolic fraction but not by that of releasable fraction
AU - Suzuki, Takeshi
AU - Kashima, Yuko
AU - Fujimoto, Kazuko
AU - Kawashima, Koichiro
N1 - Funding Information:
This work wass upportedin part by a Grant-in-Aid for ScientificR esearcho n Priority Areas (No. 03263223).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/9/14
Y1 - 1992/9/14
N2 - Synthesis and release of acetylcholine (ACh) in the rat hippocampal slices were examined to clarify the mechanism of modulation of ACh synthesis. Treatment with 2-(4-phenylpiperidino)cyclohexanol (AH5183, 50 μM), an inhibitor of ACh transport from cytosol to synaptic vesicles, inhibited the increase in ACh content of the membrane-bound fraction which is readily releasable, but did not affect the cytosolic ACh content. Under these conditions, the total ACh content reached a plateau value. These results indicate that ACh synthesis is modulated by cytosolic ACh content but not by the vesicular fraction.
AB - Synthesis and release of acetylcholine (ACh) in the rat hippocampal slices were examined to clarify the mechanism of modulation of ACh synthesis. Treatment with 2-(4-phenylpiperidino)cyclohexanol (AH5183, 50 μM), an inhibitor of ACh transport from cytosol to synaptic vesicles, inhibited the increase in ACh content of the membrane-bound fraction which is readily releasable, but did not affect the cytosolic ACh content. Under these conditions, the total ACh content reached a plateau value. These results indicate that ACh synthesis is modulated by cytosolic ACh content but not by the vesicular fraction.
KW - AH5183
KW - Acetylcholine release
KW - Acetylcholine synthesis
KW - Hippocampus
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U2 - 10.1016/0304-3940(92)90732-M
DO - 10.1016/0304-3940(92)90732-M
M3 - Article
C2 - 1436692
AN - SCOPUS:0026666241
SN - 0304-3940
VL - 144
SP - 127
EP - 129
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -