Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

Norio Tanahashi; Yasuo Fukuuchi; Minoru Tomita; Yutaka Tomita; Koji Inoue; Hideki Satoh; Takato Abe

doi:10.1016/S0304-3940(01)01608-1

Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

Norio Tanahashi, Yasuo Fukuuchi, Minoru Tomita, Yutaka Tomita, Koji Inoue, Hideki Satoh, Takato Abe

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 μM) were perfused over HBEC at a low shear rate of 10 s^-1 for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 μM) plus TAK-029 (GPIIb/IIIa antagonist; 1 μM) was perfused over HBEC for 30 min and washed out. Anti-GPIbα antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa.

Original language	English
Pages (from-to)	33-36
Number of pages	4
Journal	Neuroscience Letters
Volume	301
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(01)01608-1
Publication status	Published - 2001 Mar 23
Externally published	Yes

Keywords

GPIIb/IIIa
GPIbα
Human brain microvascular endothelial cells
Platelet adhesion
Platelet aggregation

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(01)01608-1

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title = "Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa",

abstract = "Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 μM) were perfused over HBEC at a low shear rate of 10 s-1 for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 μM) plus TAK-029 (GPIIb/IIIa antagonist; 1 μM) was perfused over HBEC for 30 min and washed out. Anti-GPIbα antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa.",

keywords = "GPIIb/IIIa, GPIbα, Human brain microvascular endothelial cells, Platelet adhesion, Platelet aggregation",

author = "Norio Tanahashi and Yasuo Fukuuchi and Minoru Tomita and Yutaka Tomita and Koji Inoue and Hideki Satoh and Takato Abe",

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T1 - Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

AU - Tanahashi, Norio

AU - Fukuuchi, Yasuo

AU - Tomita, Minoru

AU - Tomita, Yutaka

AU - Inoue, Koji

AU - Satoh, Hideki

AU - Abe, Takato

PY - 2001/3/23

Y1 - 2001/3/23

N2 - Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 μM) were perfused over HBEC at a low shear rate of 10 s-1 for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 μM) plus TAK-029 (GPIIb/IIIa antagonist; 1 μM) was perfused over HBEC for 30 min and washed out. Anti-GPIbα antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa.

AB - Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 μM) were perfused over HBEC at a low shear rate of 10 s-1 for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 μM) plus TAK-029 (GPIIb/IIIa antagonist; 1 μM) was perfused over HBEC for 30 min and washed out. Anti-GPIbα antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa.

KW - GPIIb/IIIa

KW - GPIbα

KW - Human brain microvascular endothelial cells

KW - Platelet adhesion

KW - Platelet aggregation

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Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

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