TY - JOUR
T1 - Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins
AU - Nishiyama, Keita
AU - Nakamata, Koichi
AU - Ueno, Shintaro
AU - Terao, Akari
AU - Aryantini, Ni Putu Desy
AU - Sujaya, I. Nengah
AU - Fukuda, Kenji
AU - Urashima, Tadasu
AU - Yamamoto, Yuji
AU - Mukai, Takao
N1 - Funding Information:
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) under Grant-in-Aid for Scientific Research C (24580397) and Kitasato Research Project for Lactic Acid Bacteria.
Publisher Copyright:
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2015
Y1 - 2015
N2 - We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.
AB - We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wildtype strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.
KW - Bacterial adhesion
KW - Extracellular matrix (ECM) proteins
KW - Lactobacillus rhamnosus
KW - Mucus-binding factor
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U2 - 10.1080/09168451.2014.972325
DO - 10.1080/09168451.2014.972325
M3 - Article
C2 - 25351253
AN - SCOPUS:84952714296
SN - 0916-8451
VL - 79
SP - 271
EP - 279
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 2
ER -