TY - JOUR
T1 - Adsorption-Desorption Control of Fibronectin in Real Time at the Liquid/Polymer Interface on a Quartz Crystal Microbalance by Thermoresponsivity
AU - Li, Jiatu
AU - Kaku, Taisei
AU - Tokura, Yuki
AU - Matsukawa, Ko
AU - Homma, Kenta
AU - Nishimoto, Taihei
AU - Hiruta, Yuki
AU - Akimoto, Aya Mizutani
AU - Nagase, Kenichi
AU - Kanazawa, Hideko
AU - Shiratori, Seimei
N1 - Funding Information:
*E-mail: shiratori@appi.keio.ac.jp. ORCID Yuki Hiruta: 0000-0001-7303-4189 Kenichi Nagase: 0000-0002-6575-0107 Hideko Kanazawa: 0000-0003-2550-470X Seimei Shiratori: 0000-0001-9807-3555 Author Contributions A.A. and S.S. proposed the research. J.L. and S.S. designed the experiment. J.L. conducted the experiment, analyzed the data, and wrote a paper. T.K., Y.T., K.M., K.H., T.N., Y.H., and A.A. provided experimental support and support in data analysis. A.A., K.N., H.K., and S.S. supervised the project, provided scientific advice, and commented on the manuscript. Funding This work was supported by JST SENTAN (Grant No. YYN6031). Notes The authors declare no competing financial interest.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/4/8
Y1 - 2019/4/8
N2 - The cell manipulation technique using thermoresponsive polymers is currently attracting much attention for applications in the medical field. To achieve arbitrary and accurate cell control, it is necessary to intensely research fibronectin behavior. A smart surface, which has thermoresponsive wettability and which can adsorb or desorb fibronectin repeatedly without the presence of cells, was fabricated by an electrospinning method. The fabricated coating changed its structure as the temperature was changed, and this transformation could substitute for the pulling force generated by the cytoskeletal contraction of cells. Moreover, a coated quartz crystal microbalance was able to detect the fibronectin behavior as frequency shifts, which could be used in the estimation of the mass shift with the aid of suitable equations. This coating and measurement system can contribute greatly not only to the development in the medical field centered on biomaterial manipulation technologies, but also to the improvement of medical instruments.
AB - The cell manipulation technique using thermoresponsive polymers is currently attracting much attention for applications in the medical field. To achieve arbitrary and accurate cell control, it is necessary to intensely research fibronectin behavior. A smart surface, which has thermoresponsive wettability and which can adsorb or desorb fibronectin repeatedly without the presence of cells, was fabricated by an electrospinning method. The fabricated coating changed its structure as the temperature was changed, and this transformation could substitute for the pulling force generated by the cytoskeletal contraction of cells. Moreover, a coated quartz crystal microbalance was able to detect the fibronectin behavior as frequency shifts, which could be used in the estimation of the mass shift with the aid of suitable equations. This coating and measurement system can contribute greatly not only to the development in the medical field centered on biomaterial manipulation technologies, but also to the improvement of medical instruments.
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U2 - 10.1021/acs.biomac.9b00121
DO - 10.1021/acs.biomac.9b00121
M3 - Article
C2 - 30785729
AN - SCOPUS:85062881774
SN - 1525-7797
VL - 20
SP - 1748
EP - 1755
JO - Biomacromolecules
JF - Biomacromolecules
IS - 4
ER -