Aerotaxis and aerokinesis of Dictyostelium discoideum under hypoxic microenvironments

S. Hirose, J. P. Rieu, C. Anjard, O. Cochet-Escartin, H. Kikuchi, K. Funamoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Although spatiotemporal changes of oxygen in a microenvironment are known to affect the cellular dynamics of various eukaryotes, the details are not fully understood. Here, we describe the aerotaxis and aerokinesis of Dictyostelium discoideum (Dd), which has long been employed as a model organism for eukaryotic cells. We developed a microfluidic device capable of time-lapse observation of cultured cells while controlling oxygen concentrations in microchannels. Migratory behaviors of Dd were observed and quantitatively evaluated under an oxygen concentration gradient from 0% to 21% O 2 , as well as in various uniform oxygen conditions. In a hypoxic region within the oxygen concentration gradient, Dd migrated toward regions of higher oxygen concentration at increased velocity, which was independent of cell density. Observed under uniform oxygen concentrations of 1%, 2%, 3%, and 21%, the migration velocity of Dd increased significantly in hypoxic environments of 2% O 2 or less. Thus, Dd shows aerotaxis, directed by the oxygen concentration gradient, and simultaneously shows aerokinesis, changing the migration velocity according to the oxygen concentration itself.

Original languageEnglish
Title of host publication43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1187-1190
Number of pages4
ISBN (Electronic)9781728111797
DOIs
Publication statusPublished - 2021
Externally publishedYes
Event43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021 - Virtual, Online, Mexico
Duration: 2021 Nov 12021 Nov 5

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021
Country/TerritoryMexico
CityVirtual, Online
Period21/11/121/11/5

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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