Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle

Kaori Umehara; Kazunobu Toshima; Haruma Kawaguchi

Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle

Kaori Umehara, Kazunobu Toshima, Haruma Kawaguchi

Department of Applied Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.

Original language	English
Number of pages	1
Publication status	Published - 2006 Oct 19
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Other

Other	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Affinity particle
Bulge DNA
DNA discrimination
Oligolysine
SPR (surface plasmon resonance) sensor

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle",

abstract = "We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.",

keywords = "Affinity particle, Bulge DNA, DNA discrimination, Oligolysine, SPR (surface plasmon resonance) sensor",

author = "Kaori Umehara and Kazunobu Toshima and Haruma Kawaguchi",

year = "2006",

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day = "19",

language = "English",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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T1 - Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle

AU - Umehara, Kaori

AU - Toshima, Kazunobu

AU - Kawaguchi, Haruma

PY - 2006/10/19

Y1 - 2006/10/19

N2 - We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.

AB - We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.

KW - Affinity particle

KW - Bulge DNA

KW - DNA discrimination

KW - Oligolysine

KW - SPR (surface plasmon resonance) sensor

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M3 - Paper

AN - SCOPUS:33749842350

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Affinity discrimination of DNA on SPR sensor using oligolysine-immobilized particle

Abstract

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Keywords

ASJC Scopus subject areas

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