Air leak seal for lung dissection plane with diode laser irradiation: Monitoring heat-denature with auto-fluorescence

Maya Gotoh, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We studied the monitoring of heat-denature by auto-fluorescence spectrum from lung dissection plane during laser air leak sealing procedure. In order to seal the air leakage from lung in thoracotomy, we proposed novel laser sealing method with the combination of the diode laser (810nm wavelength) irradiation and indocyanine green staining (peak absorption wavelength: 805 nm). This sealing method is expected to preserve the postoperative ventilatory capacity and achieve minimally invasive surgery. We previously reported that this laser sealing only requires thin sealing margin (less than 300 μm in thickness) compared with that of the suturing or stapling. The most serious issue on the laser air leak sealing might be re-air-leakage due to rigid surface layer caused by excessive heat-denature, such as carbonization. We should achieve laser air leak sealing minimizing the degree of heat-denature. Dissection planes of isolated porcine lung with / without the diode laser irradiation were prepared as samples. We measured the auto-fluorescence from these samples using a spectrometer. When the diode laser was irradiated with 400J/cm2, the surface of diode laser irradiated lung was fully carbonized. The ratio of auto-fluorescence emission of 450nm / 500 nm, with 280nm excitation wavelength was decreased less than 50 % of initial value. That of 600 nm / 500 nm was increased over 700 % of initial value. The decreasing of the 450 nm auto-fluorescence intensity might be attributed to the heat-denaturing of the interstitial collagen in lung. However increaseing of the 600 nm didn't specify the origins, we suppose it might be originated from, heat-denature substance, like carbonization. We could establish the useful monitoring for lung heat-denaturing with simple methodology. We think the auto-fluorescence measurement can be helpful not only for understanding the sealing mechanism, but also for controlling the degree of heat-denaturing during the procedure.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XIX
Publication statusPublished - 2008 Apr 21
EventOptical Interactions with Tissue and Cells XIX - San Jose, CA, United States
Duration: 2008 Jan 212008 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Interactions with Tissue and Cells XIX
Country/TerritoryUnited States
CitySan Jose, CA


  • Air leak seal
  • Auto-fluorescence
  • Diode laser
  • Heat denature
  • Indocyanine green
  • Thoracotomy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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