TY - GEN
T1 - Aligned free-standing muscle fibers connected with neurons
AU - Morimoto, Y.
AU - Kato-Negishi, M.
AU - Onoe, H.
AU - Takeuchi, S.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - We propose aligned free-standing skeletal muscle fibers connecting with neurons. By confining skeletal cells, C2C12, with collagen gel into polydimethylsiloxane (PDMS) trenches, we successfully formed the muscle fibers that are highly oriented to the fiber direction. We cultured the free-standing muscle fibers maintaining their tension since the edge of the fibers was fixed to glass substrates. Furthermore, we can culture the muscle fibers with rat primary neurons, and the neurons successfully connect with the muscle fibers. We believe that the muscle fibers connected with the neurons have a potential to contract by the nerve impulse.
AB - We propose aligned free-standing skeletal muscle fibers connecting with neurons. By confining skeletal cells, C2C12, with collagen gel into polydimethylsiloxane (PDMS) trenches, we successfully formed the muscle fibers that are highly oriented to the fiber direction. We cultured the free-standing muscle fibers maintaining their tension since the edge of the fibers was fixed to glass substrates. Furthermore, we can culture the muscle fibers with rat primary neurons, and the neurons successfully connect with the muscle fibers. We believe that the muscle fibers connected with the neurons have a potential to contract by the nerve impulse.
KW - 3D tissue engineering
KW - Muscle contraction
KW - Muscle fiber
KW - Neuron-muscle co-culture
KW - PDMS stamp
UR - http://www.scopus.com/inward/record.url?scp=84883772137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883772137&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883772137
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 789
EP - 791
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -