TY - JOUR
T1 - Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model
AU - Garcia-Contreras, Rene
AU - Sugimoto, Masahiro
AU - Umemura, Naoki
AU - Kaneko, Miku
AU - Hatakeyama, Yoko
AU - Soga, Tomoyoshi
AU - Tomita, Masaru
AU - Scougall-Vilchis, Rogelio J.
AU - Contreras-Bulnes, Rosalia
AU - Nakajima, Hiroshi
AU - Sakagami, Hiroshi
N1 - Funding Information:
We are very grateful Ms. Yumiko Kanda for her excellent technical assistant with operating the transmission electron microscope. This work was supported by Grant-in-Aid for Challenging Exploratory Research from The Ministry of Education, Culture, Sports, Science and Technology (Sakagami H. 25670897 ), research funds from Meikai University School of Dentistry and a research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO2 NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1β alone or in combination with TiO2 NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO2 NPs into vacuoles of the cells. TiO2 NPs significantly enhanced the IL-1β-induced prostaglandin E2 production and COX-1 and COX-2 protein expression. IL-1β reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO2 NPs further augmented these IL-1β-induced metabolic changes, recommending careful use of dental materials containing TiO2 NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO2 NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation.
AB - Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO2 NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1β alone or in combination with TiO2 NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO2 NPs into vacuoles of the cells. TiO2 NPs significantly enhanced the IL-1β-induced prostaglandin E2 production and COX-1 and COX-2 protein expression. IL-1β reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO2 NPs further augmented these IL-1β-induced metabolic changes, recommending careful use of dental materials containing TiO2 NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO2 NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation.
KW - Human gingival fibroblast
KW - Inerleukin-1β
KW - Inflammation
KW - Metabolomics
KW - Titanium dioxide nanoparticle
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U2 - 10.1016/j.biomaterials.2015.03.059
DO - 10.1016/j.biomaterials.2015.03.059
M3 - Article
C2 - 25913073
AN - SCOPUS:84928944403
SN - 0142-9612
VL - 57
SP - 33
EP - 40
JO - Biomaterials
JF - Biomaterials
ER -