Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model

Rene Garcia-Contreras, Masahiro Sugimoto, Naoki Umemura, Miku Kaneko, Yoko Hatakeyama, Tomoyoshi Soga, Masaru Tomita, Rogelio J. Scougall-Vilchis, Rosalia Contreras-Bulnes, Hiroshi Nakajima, Hiroshi Sakagami

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO2 NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1β alone or in combination with TiO2 NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO2 NPs into vacuoles of the cells. TiO2 NPs significantly enhanced the IL-1β-induced prostaglandin E2 production and COX-1 and COX-2 protein expression. IL-1β reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO2 NPs further augmented these IL-1β-induced metabolic changes, recommending careful use of dental materials containing TiO2 NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO2 NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
Publication statusPublished - 2015 Jul 1


  • Human gingival fibroblast
  • Inerleukin-1β
  • Inflammation
  • Metabolomics
  • Titanium dioxide nanoparticle

ASJC Scopus subject areas

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials


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