Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells

Masayuki Fujita; Rei Takahashi; Kazuhiro Kitada; Rei Watanabe; Sohei Kitazawa; Faramarz Ashoori; Ping Liang; Hideyuki Saya; Tadao Serikawa; Sakan Maeda

doi:10.1016/S0304-3835(96)04544-2

Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells

Masayuki Fujita, Rei Takahashi, Kazuhiro Kitada, Rei Watanabe, Sohei Kitazawa, Faramarz Ashoori, Ping Liang, Hideyuki Saya, Tadao Serikawa, Sakan Maeda

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An alternative splicing of the rat erythropoietin receptor (EpoR) gene; was identified in normal and erythroleukemia cells. A 105 bp insert was found at a region corresponding to the extracellular domain of EpoR. The alternative transcript was translated to a soluble EpoR (EpoR-S) expressed in spleen, bone marrow, and cultured erythroleukemia cells in addition to the full-length EpoR (EpoR-F). One of the rat erythroleukemia sublines, K4DT, which partially lost erythroid phenotypes and manifested monocyte/macrophage characteristics also lacked EpoR-S expression. Thus, expression of EpoR-S may play an important role in differentiation of rat erythroid cells.

Original language	English
Pages (from-to)	47-55
Number of pages	9
Journal	Cancer Letters
Volume	112
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(96)04544-2
Publication status	Published - 1997 Jan 15
Externally published	Yes

Keywords

Alternative splicing
Differentiation
Erythropoietin receptor
Leukemia

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(96)04544-2

Cite this

@article{d2f6aad993db48e88eb77c97c6c9fb76,

title = "Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells",

abstract = "An alternative splicing of the rat erythropoietin receptor (EpoR) gene; was identified in normal and erythroleukemia cells. A 105 bp insert was found at a region corresponding to the extracellular domain of EpoR. The alternative transcript was translated to a soluble EpoR (EpoR-S) expressed in spleen, bone marrow, and cultured erythroleukemia cells in addition to the full-length EpoR (EpoR-F). One of the rat erythroleukemia sublines, K4DT, which partially lost erythroid phenotypes and manifested monocyte/macrophage characteristics also lacked EpoR-S expression. Thus, expression of EpoR-S may play an important role in differentiation of rat erythroid cells.",

keywords = "Alternative splicing, Differentiation, Erythropoietin receptor, Leukemia",

author = "Masayuki Fujita and Rei Takahashi and Kazuhiro Kitada and Rei Watanabe and Sohei Kitazawa and Faramarz Ashoori and Ping Liang and Hideyuki Saya and Tadao Serikawa and Sakan Maeda",

year = "1997",

month = jan,

day = "15",

doi = "10.1016/S0304-3835(96)04544-2",

language = "English",

volume = "112",

pages = "47--55",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells

AU - Fujita, Masayuki

AU - Takahashi, Rei

AU - Kitada, Kazuhiro

AU - Watanabe, Rei

AU - Kitazawa, Sohei

AU - Ashoori, Faramarz

AU - Liang, Ping

AU - Saya, Hideyuki

AU - Serikawa, Tadao

AU - Maeda, Sakan

PY - 1997/1/15

Y1 - 1997/1/15

N2 - An alternative splicing of the rat erythropoietin receptor (EpoR) gene; was identified in normal and erythroleukemia cells. A 105 bp insert was found at a region corresponding to the extracellular domain of EpoR. The alternative transcript was translated to a soluble EpoR (EpoR-S) expressed in spleen, bone marrow, and cultured erythroleukemia cells in addition to the full-length EpoR (EpoR-F). One of the rat erythroleukemia sublines, K4DT, which partially lost erythroid phenotypes and manifested monocyte/macrophage characteristics also lacked EpoR-S expression. Thus, expression of EpoR-S may play an important role in differentiation of rat erythroid cells.

AB - An alternative splicing of the rat erythropoietin receptor (EpoR) gene; was identified in normal and erythroleukemia cells. A 105 bp insert was found at a region corresponding to the extracellular domain of EpoR. The alternative transcript was translated to a soluble EpoR (EpoR-S) expressed in spleen, bone marrow, and cultured erythroleukemia cells in addition to the full-length EpoR (EpoR-F). One of the rat erythroleukemia sublines, K4DT, which partially lost erythroid phenotypes and manifested monocyte/macrophage characteristics also lacked EpoR-S expression. Thus, expression of EpoR-S may play an important role in differentiation of rat erythroid cells.

KW - Alternative splicing

KW - Differentiation

KW - Erythropoietin receptor

KW - Leukemia

UR - http://www.scopus.com/inward/record.url?scp=0031014041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031014041&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(96)04544-2

DO - 10.1016/S0304-3835(96)04544-2

M3 - Article

C2 - 9029168

AN - SCOPUS:0031014041

SN - 0304-3835

VL - 112

SP - 47

EP - 55

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this