Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs

Tomoka Yoshii, Mai Mochida, Kogi Kaizu, Yasuki Soda, Kazuyoshi Kanamori, Kazuki Nakanishi, Takaaki Sato, Hiroaki Imai, Daniel Citterio, Yuki Hiruta

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


A way to utilize eggshells is desired because most of the eggshell waste is disposed of in landfills. Despite eggshells having attractive features such as a thermodynamically stable porous crystalline structure, their effective utilization as a functional material has yet to be demonstrated. Herein, we developed an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) based on eggshells. Nanocrystals and biopolymers present on eggshell powder were removed by surface treatment with buffered ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite solutions, resulting in bare calcium carbonate with a uniform porous surface. The obtained particles were modified with poly(maleic acid-alt-1-octadecene) (PMAcO) having hydrophobic alkyl chains and anionic carboxylate groups. The prepared PMAcO-modified eggshell-based material was applied to an HPLC column, and its performance is evaluated. The column succeeded in the separation of alkylbenzenes, steroids, and diastereomers. It was also used for the separation of basic tricyclic antidepressants in an alkaline mobile phase, showing excellent retention capacity and alkali stability. This study demonstrates the potential of eggshell utilization, providing not only a practical stationary phase for HPLC but also a solution for recycling calcium carbonate-based biomineral waste.

Original languageEnglish
Pages (from-to)6949-6957
Number of pages9
JournalACS Applied Polymer Materials
Issue number10
Publication statusPublished - 2022 Oct 14


  • alternating copolymer
  • biomineral
  • calcium carbonate
  • chromatography
  • food waste

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Polymers and Plastics
  • Organic Chemistry


Dive into the research topics of 'Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs'. Together they form a unique fingerprint.

Cite this