TY - JOUR
T1 - Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs
AU - Yoshii, Tomoka
AU - Mochida, Mai
AU - Kaizu, Kogi
AU - Soda, Yasuki
AU - Kanamori, Kazuyoshi
AU - Nakanishi, Kazuki
AU - Sato, Takaaki
AU - Imai, Hiroaki
AU - Citterio, Daniel
AU - Hiruta, Yuki
N1 - Funding Information:
This research was supported by Adaptable and Seamless Technology transfer Program through Target-driven R&D (A-STEP) from Japan Science and Technology Agency (JST) Grant Number JPMJTM20D7, Mizuho Foundation for the Promotion of Sciences, and Mukai Science and Technology Foundation. The authors thank Dr. Kei Morisato of Kyoto University Dr. Akihiro Sakama, and Kana Shibuya of Keio University for their support with mercury porosimetry, NMR analysis, and synthesis of dehydrostemonine respectively.
Funding Information:
This research was supported by Adaptable and Seamless Technology transfer Program through Target-driven R&D (A-STEP) from Japan Science and Technology Agency (JST) Grant Number JPMJTM20D7, Mizuho Foundation for the Promotion of Sciences, and Mukai Science and Technology Foundation. The authors thank Dr. Kei Morisato of Kyoto University, Dr. Akihiro Sakama, and Kana Shibuya of Keio University for their support with mercury porosimetry, NMR analysis, and synthesis of dehydrostemonine, respectively.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/10/14
Y1 - 2022/10/14
N2 - A way to utilize eggshells is desired because most of the eggshell waste is disposed of in landfills. Despite eggshells having attractive features such as a thermodynamically stable porous crystalline structure, their effective utilization as a functional material has yet to be demonstrated. Herein, we developed an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) based on eggshells. Nanocrystals and biopolymers present on eggshell powder were removed by surface treatment with buffered ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite solutions, resulting in bare calcium carbonate with a uniform porous surface. The obtained particles were modified with poly(maleic acid-alt-1-octadecene) (PMAcO) having hydrophobic alkyl chains and anionic carboxylate groups. The prepared PMAcO-modified eggshell-based material was applied to an HPLC column, and its performance is evaluated. The column succeeded in the separation of alkylbenzenes, steroids, and diastereomers. It was also used for the separation of basic tricyclic antidepressants in an alkaline mobile phase, showing excellent retention capacity and alkali stability. This study demonstrates the potential of eggshell utilization, providing not only a practical stationary phase for HPLC but also a solution for recycling calcium carbonate-based biomineral waste.
AB - A way to utilize eggshells is desired because most of the eggshell waste is disposed of in landfills. Despite eggshells having attractive features such as a thermodynamically stable porous crystalline structure, their effective utilization as a functional material has yet to be demonstrated. Herein, we developed an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) based on eggshells. Nanocrystals and biopolymers present on eggshell powder were removed by surface treatment with buffered ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite solutions, resulting in bare calcium carbonate with a uniform porous surface. The obtained particles were modified with poly(maleic acid-alt-1-octadecene) (PMAcO) having hydrophobic alkyl chains and anionic carboxylate groups. The prepared PMAcO-modified eggshell-based material was applied to an HPLC column, and its performance is evaluated. The column succeeded in the separation of alkylbenzenes, steroids, and diastereomers. It was also used for the separation of basic tricyclic antidepressants in an alkaline mobile phase, showing excellent retention capacity and alkali stability. This study demonstrates the potential of eggshell utilization, providing not only a practical stationary phase for HPLC but also a solution for recycling calcium carbonate-based biomineral waste.
KW - alternating copolymer
KW - biomineral
KW - calcium carbonate
KW - chromatography
KW - food waste
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U2 - 10.1021/acsapm.2c00866
DO - 10.1021/acsapm.2c00866
M3 - Article
AN - SCOPUS:85136147727
SN - 2637-6105
VL - 4
SP - 6949
EP - 6957
JO - ACS Applied Polymer Materials
JF - ACS Applied Polymer Materials
IS - 10
ER -