Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe, Rei Murakami, Hideyuki Okano

Research output: Chapter in Book/Report/Conference proceedingChapter


Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer’s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Amyloid β (Aβ)
  • Enzyme-Linked Immuno Sorbent Assay (ELISA)
  • Neuronal differentiation
  • induced pluripotent stem cell (iPSC)
  • β-Secretase
  • γ-Secretase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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