An algorithm to evaluate the number of trabecular cell layers using nucleus arrangement applied to hepatocellular carcinoma

Hideki Komagata, Naoki Kobayashi, Ayako Katoh, Yasuka Ohnuki, Masahiro Ishikawa, Kazuma Shinoda, Masahiro Yamaguchi, Tokiya Abe, Akinori Hashiguchi, Michiie Sakamoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Recent advances in information technology have improved pathological virtual-slide technology and diagnostic support system studies of pathological images. Diagnostic support systems utilize quantitative indices determined by image processing. In previous studies on diagnostic support systems, carcinomatous areas of breast or lung have been recognized by the feature quantities of nuclear sizes, complexities, and internuclear distances based on graph theory, among other features. Improving recognition accuracy is important for the addition of new feature quantities. We focused on hepatocellular carcinoma (HCC) and investigated new feature quantities of histological images of HCC. One of the most important histological features of HCC is the trabecular pattern. For diagnosing cancer, it is important to recognize the tumor cell trabeculae. We propose a new algorithm for calculating the number of cell layers in histological images of HCC in tissue sections stained by hematoxylin and eosin. For the calculation, we used a Delaunay diagram that was based on the median points of nuclei, deleted the sinusoid and fat droplet regions from the Delaunay diagram, and counted the Delaunay lines while applying a thinning algorithm. Moreover, we experimented with the calculation of the number of cell layers with our method for different histological grades of HCC. The number of cell layers discriminated tumor differentiations and Edmondson grades; therefore, our algorithm may serve as an index of HCC for diagnostic support systems.

Original languageEnglish
Title of host publicationMedical Imaging 2013
Subtitle of host publicationDigital Pathology
Publication statusPublished - 2013
EventSPIE Medical Imaging Symposium 2013: Digital Pathology - Lake Buena Vista, FL, United States
Duration: 2013 Feb 102013 Feb 11

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherSPIE Medical Imaging Symposium 2013: Digital Pathology
Country/TerritoryUnited States
CityLake Buena Vista, FL


  • Delaunay diagram
  • Digital pathology
  • Feature extraction
  • Hepatocellular carcinoma
  • Thinning algorithm

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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