TY - JOUR
T1 - An Entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from Entamoeba histolytica
AU - Tachibana, Hiroshi
AU - Yanagi, Tetsuo
AU - Pandey, Kishor
AU - Cheng, Xun Jia
AU - Kobayashi, Seiki
AU - Sherchand, Jeevan B.
AU - Kanbara, Hiroji
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science, grants from the Ministry of Health, Labour, and Welfare of Japan, and a Cooperative Research Grant 2006-18-C-4 of the Institute of Tropical Medicine, Nagasaki University.
PY - 2007/6
Y1 - 2007/6
N2 - An Entamoeba sp. strain, P19-061405, was isolated from a rhesus monkey in Nepal and characterized genetically. The strain was initially identified as Entamoeba histolytica using PCR amplification of peroxiredoxin genes. However, sequence analysis of the 18S rRNA gene showed a 0.8% difference when compared to the reference E. histolytica HM-1:IMSS human strain. Differences were also observed in the 5.8S rRNA gene and the internal transcribed spacer (ITS) regions 1 and 2, and analysis of the serine-rich protein gene from the monkey strain showed unique codon usages compared to E. histolytica isolated from humans. The amino acid sequences of two hexokinases and two glucose phosphate isomerases also differed from those of E. histolytica. Isoenzyme analyses of these enzymes in the monkey strain showed different electrophoretic mobility patterns compared with E. histolytica isolates. Analysis of peroxiredoxin genes indicated the presence of at least seven different types of protein, none of which were identical to proteins in E. histolytica. When the trophozoites from the monkey strain were inoculated into the livers of hamsters, formation of amebic abscesses was observed 7 days after the injection. These results demonstrate that the strain is genetically different from E. histolytica and is virulent. Revival of the name Entamoeba nuttalli is proposed for the organism.
AB - An Entamoeba sp. strain, P19-061405, was isolated from a rhesus monkey in Nepal and characterized genetically. The strain was initially identified as Entamoeba histolytica using PCR amplification of peroxiredoxin genes. However, sequence analysis of the 18S rRNA gene showed a 0.8% difference when compared to the reference E. histolytica HM-1:IMSS human strain. Differences were also observed in the 5.8S rRNA gene and the internal transcribed spacer (ITS) regions 1 and 2, and analysis of the serine-rich protein gene from the monkey strain showed unique codon usages compared to E. histolytica isolated from humans. The amino acid sequences of two hexokinases and two glucose phosphate isomerases also differed from those of E. histolytica. Isoenzyme analyses of these enzymes in the monkey strain showed different electrophoretic mobility patterns compared with E. histolytica isolates. Analysis of peroxiredoxin genes indicated the presence of at least seven different types of protein, none of which were identical to proteins in E. histolytica. When the trophozoites from the monkey strain were inoculated into the livers of hamsters, formation of amebic abscesses was observed 7 days after the injection. These results demonstrate that the strain is genetically different from E. histolytica and is virulent. Revival of the name Entamoeba nuttalli is proposed for the organism.
KW - Entamoeba histolytica
KW - Entamoeba nuttalli
KW - Rhesus monkey
KW - Virulency
KW - rRNA gene
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U2 - 10.1016/j.molbiopara.2007.02.006
DO - 10.1016/j.molbiopara.2007.02.006
M3 - Article
C2 - 17403547
AN - SCOPUS:34247497373
SN - 0166-6851
VL - 153
SP - 107
EP - 114
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -