An evaluation of semen processing methods for eliminating HIV-1

Hideji Hanabusa, Naoaki Kuji, Shingo Kato, Hisamichi Tagami, Satoru Kaneko, Hiroaki Tanaka, Yasunori Yoshimura

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)


Objective: Whether artificial insemination can provide adequate protection for discordant couples where the man is HIV-1 positive and the woman is HIV-1 negative is uncertain because of the paucity of HIV-1 elimination data assessing current sperm-washing techniques. We evaluated how effectively these techniques eliminate HIV-1 RNA and proviral DNA from semen. Methods: Spermatozoa were separated from semen samples from HIV-1-positive patients with haemophilia by discontinuous Percoll gradient centrifugation and the 'swim-up' method. The HIV-1 RNA and proviral DNA were measured by a highly sensitive PCR. In another test 5 x 106 copies of HIV-1 RNA (LAI strain) were added to semen from healthy donors and then assessed after single and combined procedures. Results: Swim-up processing after Percoll gradient centrifugation reduced HIV-1 RNA and HIV-1 proviral DNA in semen to undetectable levels in the original specimen. Although discontinuous and continuous Percoll gradient centrifugation respectively reduced HIV-1 RNA added to seminal plasma specimens from healthy donors to less than < 1 copy from 105 and about 1 copy per 103 pre-separation copies, the discontinuous method left detectable HIV-1 RNA and proviral DNA in one out of 12 samples from patients with HIV-1 infection (8%). HIV-1 RNA and proviral DNA were decreased to undetectable levels after adding the swim-up procedure. Conclusions: Swim-up separation following Percoll gradient centrifugation should offer adequate protection for HIV-1-discordant couples. (C) 2000 Lippincot Williams and Wilkins.

Original languageEnglish
Pages (from-to)1611-1616
Number of pages6
Issue number11
Publication statusPublished - 2000
Externally publishedYes


  • DNA
  • HIV
  • Percoll centrifugation
  • RNA
  • Semen
  • Spermatozoa
  • Swim-up
  • Transmission

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases


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