TY - JOUR
T1 - An Evolutionarily Conserved Mechanism for MicroRNA-223 Expression Revealed by MicroRNA Gene Profiling
AU - Fukao, Taro
AU - Fukuda, Yoko
AU - Kiga, Kotaro
AU - Sharif, Jafar
AU - Hino, Kimihiro
AU - Enomoto, Yutaka
AU - Kawamura, Aya
AU - Nakamura, Kaito
AU - Takeuchi, Tsutomu
AU - Tanabe, Masanobu
N1 - Funding Information:
We would like to thank Drs. Michael Lanotte and Masahiro Kizaki for providing us NB4 cells and Prof. Takatsu and Dr. Takaki for WEHI-231 cells. This work is supported by grants from The Sumitomo Foundation (T.F.), Schering Japan (T.F.), and the Keio Gijuku Fukuzawa Memorial Fund (M.T.).
PY - 2007/5/4
Y1 - 2007/5/4
N2 - Many microRNAs (miRNAs) are evolutionarily conserved and have intriguing expression patterns. Tissue and/or time-specific expressions of some miRNAs are presumably controlled by unique cis-acting regulatory elements that coevolved with the miRNA sequences. Exploiting bioinformatics, we identified several miRNAs whose primary transcripts could be regulated by conserved genomic elements proximal to their transcription start sites. Such miRNAs include microRNA-223 (miR-223), which is reportedly controlled by a unique regulatory mechanism during granulopoiesis. Here, we define a mechanism distinct from that previously proposed to regulate miR-223 expression. We find that the mir-223 gene resembles a "myeloid gene" and might be driven by the myeloid transcription factors, PU.1 and C/EBPs. This mechanism is specified by the conserved proximal cis-regulatory element and might be common among different species. Hence, it needs to be considered that two distinct mechanisms that would play critical roles in myeloid functions and differentiation are actually concerned with the regulation of miR-223.
AB - Many microRNAs (miRNAs) are evolutionarily conserved and have intriguing expression patterns. Tissue and/or time-specific expressions of some miRNAs are presumably controlled by unique cis-acting regulatory elements that coevolved with the miRNA sequences. Exploiting bioinformatics, we identified several miRNAs whose primary transcripts could be regulated by conserved genomic elements proximal to their transcription start sites. Such miRNAs include microRNA-223 (miR-223), which is reportedly controlled by a unique regulatory mechanism during granulopoiesis. Here, we define a mechanism distinct from that previously proposed to regulate miR-223 expression. We find that the mir-223 gene resembles a "myeloid gene" and might be driven by the myeloid transcription factors, PU.1 and C/EBPs. This mechanism is specified by the conserved proximal cis-regulatory element and might be common among different species. Hence, it needs to be considered that two distinct mechanisms that would play critical roles in myeloid functions and differentiation are actually concerned with the regulation of miR-223.
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U2 - 10.1016/j.cell.2007.02.048
DO - 10.1016/j.cell.2007.02.048
M3 - Article
C2 - 17482553
AN - SCOPUS:34247483919
SN - 0092-8674
VL - 129
SP - 617
EP - 631
JO - Cell
JF - Cell
IS - 3
ER -