An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Li Linxiang; Yoshihiro Abe; Yukiko Nagasawa; Risa Kudo; Noriko Usui; Kazuhiro Imai; Tadahiko Mashino; Masataka Mochizuki; Naoki Miyata

doi:10.1002/bmc.339

An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Li Linxiang, Yoshihiro Abe, Yukiko Nagasawa, Risa Kudo, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

Research output: Contribution to journal › Article › peer-review

109 Citations (Scopus)

Abstract

An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

Original language	English
Pages (from-to)	470-474
Number of pages	5
Journal	Biomedical Chromatography
Volume	18
Issue number	7
DOIs	https://doi.org/10.1002/bmc.339
Publication status	Published - 2004 Sept

Keywords

2-Hydroxyterephthalic acid
Fenton reaction
Fluorescence detection
HPLC
Hydroxyl radical
Terephthalic acid

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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10.1002/bmc.339

Cite this

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title = "An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid",

abstract = "An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).",

keywords = "2-Hydroxyterephthalic acid, Fenton reaction, Fluorescence detection, HPLC, Hydroxyl radical, Terephthalic acid",

author = "Li Linxiang and Yoshihiro Abe and Yukiko Nagasawa and Risa Kudo and Noriko Usui and Kazuhiro Imai and Tadahiko Mashino and Masataka Mochizuki and Naoki Miyata",

year = "2004",

month = sep,

doi = "10.1002/bmc.339",

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T1 - An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

AU - Linxiang, Li

AU - Abe, Yoshihiro

AU - Nagasawa, Yukiko

AU - Kudo, Risa

AU - Usui, Noriko

AU - Imai, Kazuhiro

AU - Mashino, Tadahiko

AU - Mochizuki, Masataka

AU - Miyata, Naoki

PY - 2004/9

Y1 - 2004/9

N2 - An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

AB - An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

KW - 2-Hydroxyterephthalic acid

KW - Fenton reaction

KW - Fluorescence detection

KW - HPLC

KW - Hydroxyl radical

KW - Terephthalic acid

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An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Abstract

Keywords

ASJC Scopus subject areas

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