An improved retroviral vector for assaying promoter activity. Analysis of promoter interference in pIP211 vector

Kazunori Nakajima; Kazuhiro Ikenaka; Kensuke Nakahira; Noriyuki Morita; Katsuhiko Mikoshiba

doi:10.1016/0014-5793(93)81148-S

An improved retroviral vector for assaying promoter activity. Analysis of promoter interference in pIP211 vector

Kazunori Nakajima, Kazuhiro Ikenaka, Kensuke Nakahira, Noriyuki Morita, Katsuhiko Mikoshiba

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We recently developed a novel promoter assay system using a retroviral vector (pIP200 series). Transcription from the internal promoter, which had been inserted for the promoter assay, was shown to be interfered with by transcription from the upstream long terminal repeat (LTR). Here we report a new high-titer 'self-inactivating' vector, in which transcription interference was virtually eliminated. This new vector was constructed by introducing only a very minor mutation into the 'TATA box' in the 3'-LTR. This mutation was successfully transferred to the 5'-LTR after reverse transcription, yielding a provirus incapable of transcribing viral RNA. The viral titer was not reduced by the mutation, permitting general application of this virus.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	FEBS Letters
Volume	315
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)81148-S
Publication status	Published - 1993 Jan 4
Externally published	Yes

Keywords

Fluorescein-di-β-d-galactopyranoside
Promoter interference
Retroviral vector

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(93)81148-S

Cite this

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abstract = "We recently developed a novel promoter assay system using a retroviral vector (pIP200 series). Transcription from the internal promoter, which had been inserted for the promoter assay, was shown to be interfered with by transcription from the upstream long terminal repeat (LTR). Here we report a new high-titer 'self-inactivating' vector, in which transcription interference was virtually eliminated. This new vector was constructed by introducing only a very minor mutation into the 'TATA box' in the 3'-LTR. This mutation was successfully transferred to the 5'-LTR after reverse transcription, yielding a provirus incapable of transcribing viral RNA. The viral titer was not reduced by the mutation, permitting general application of this virus.",

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AU - Nakajima, Kazunori

AU - Ikenaka, Kazuhiro

AU - Nakahira, Kensuke

AU - Morita, Noriyuki

AU - Mikoshiba, Katsuhiko

PY - 1993/1/4

Y1 - 1993/1/4

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AB - We recently developed a novel promoter assay system using a retroviral vector (pIP200 series). Transcription from the internal promoter, which had been inserted for the promoter assay, was shown to be interfered with by transcription from the upstream long terminal repeat (LTR). Here we report a new high-titer 'self-inactivating' vector, in which transcription interference was virtually eliminated. This new vector was constructed by introducing only a very minor mutation into the 'TATA box' in the 3'-LTR. This mutation was successfully transferred to the 5'-LTR after reverse transcription, yielding a provirus incapable of transcribing viral RNA. The viral titer was not reduced by the mutation, permitting general application of this virus.

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KW - Retroviral vector

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An improved retroviral vector for assaying promoter activity. Analysis of promoter interference in pIP211 vector

Abstract

Keywords

ASJC Scopus subject areas

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