An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region

Kazutoyo Osoegawa; Rie Susukida; Saishi Okano; Jun Kudoh; Shinsei Minoshima; Nobuyoshi Shimizu; Pieter J. De Jong; Juergen Groet; Jane Ives; Hans Lehrach; Dean Nizetic; Eiichi Soeda

doi:10.1006/geno.1996.0132

An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region

Kazutoyo Osoegawa, Rie Susukida, Saishi Okano, Jun Kudoh, Shinsei Minoshima, Nobuyoshi Shimizu, Pieter J. De Jong, Juergen Groet, Jane Ives, Hans Lehrach, Dean Nizetic, Eiichi Soeda

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high- resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites, etc. Furthermore, our results have exemplified a strategy for the completion of the chromosome 21 map to sequencing.

Original language	English
Pages (from-to)	375-387
Number of pages	13
Journal	Genomics
Volume	32
Issue number	3
DOIs	https://doi.org/10.1006/geno.1996.0132
Publication status	Published - 1996 Mar 15
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1006/geno.1996.0132

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title = "An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region",

abstract = "The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high- resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites, etc. Furthermore, our results have exemplified a strategy for the completion of the chromosome 21 map to sequencing.",

author = "Kazutoyo Osoegawa and Rie Susukida and Saishi Okano and Jun Kudoh and Shinsei Minoshima and Nobuyoshi Shimizu and {De Jong}, {Pieter J.} and Juergen Groet and Jane Ives and Hans Lehrach and Dean Nizetic and Eiichi Soeda",

note = "Funding Information: Also, we thank T. Shimokawa and H. Kishida for excellent technical assistance in the initial stage of this work. This work was accomplished under the auspices of RIKEN Project ``Human Genome Organization'' and was also supported by Special Coordination Funds of the Science and Technology Agency of the Japanese Government, a Grant-in-Aid for Creative Basic Research (Human Genome Program), Grant-in-Aid for Scienti{\textregistered}c Research from the Ministry of Education, Science and Culture of Japan, and partly by Grant GENO-CT93-0015 from the Commission of European Communities.",

year = "1996",

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doi = "10.1006/geno.1996.0132",

language = "English",

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AU - Osoegawa, Kazutoyo

AU - Susukida, Rie

AU - Okano, Saishi

AU - Kudoh, Jun

AU - Minoshima, Shinsei

AU - Shimizu, Nobuyoshi

AU - De Jong, Pieter J.

AU - Groet, Juergen

AU - Ives, Jane

AU - Lehrach, Hans

AU - Nizetic, Dean

AU - Soeda, Eiichi

N1 - Funding Information: Also, we thank T. Shimokawa and H. Kishida for excellent technical assistance in the initial stage of this work. This work was accomplished under the auspices of RIKEN Project ``Human Genome Organization'' and was also supported by Special Coordination Funds of the Science and Technology Agency of the Japanese Government, a Grant-in-Aid for Creative Basic Research (Human Genome Program), Grant-in-Aid for Scienti®c Research from the Ministry of Education, Science and Culture of Japan, and partly by Grant GENO-CT93-0015 from the Commission of European Communities.

PY - 1996/3/15

Y1 - 1996/3/15

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An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region

Abstract

ASJC Scopus subject areas

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