An isolated tumor perfusion model in mice

Annique M.M.J. Duyverman, Mitsutomo Kohno, Sylvie Roberge, Dai Fukumura, Dan G. Duda, Rakesh K. Jain

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2-10 weeks, depending on the growth rate of the primary tumor.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalNature Protocols
Issue number4
Publication statusPublished - 2012 Apr
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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