Analysis of melatonin using a pH- and temperature-responsive aqueous chromatography system

Eri Ayano, Yusuke Suzuki, Masahito Kanezawa, Chikako Sakamoto, Yuko Morita-Murase, Yoshiko Nagata, Hideko Kanazawa, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalJournal of Chromatography A
Issue number1-2 SPEC. ISS.
Publication statusPublished - 2007 Jul 13


  • HPLC
  • Intracerebral hormone
  • Melatonin
  • Poly(N-isopropylacrylamide)
  • pH- and temperature-responsive chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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