TY - JOUR
T1 - Analysis of peptides and proteins by temperature-responsive chromatographic system using N-isopropylacrylamide polymer-modified columns
AU - Kanazawa, Hideko
AU - Yamamoto, Kazuo
AU - Kashiwase, Yuki
AU - Matsushima, Yoshikazu
AU - Takai, Nobuharu
AU - Kikuchi, Akihiko
AU - Sakurai, Yasuhisa
AU - Okano, Teruo
PY - 1997/6
Y1 - 1997/6
N2 - A new method of HPLC using packing materials modified with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), was developed. Homogeneous PIPAAm polymer and its copolymer with butyl methacrylate (BMA) were synthesized and grafted to aminopropyl silica by activated ester-amine coupling and they were used as packing materials. The surface properties and functions of the stationary phases are controlled by external temperature. Isocratic elution by aqueous mobile phase alone is the basis for separation of peptides and protein. The separation of the mixture of three peptides, insulin chain A and B and β-endorphin fragment 1-27 was achieved by changing the column temperature with 0.9% NaCl aqueous solution as the sole eluent. Retention of peptides and proteins was controlled both by column temperature and by NaCl concentration in the aqueous mobile phases in this chromatographic system.
AB - A new method of HPLC using packing materials modified with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), was developed. Homogeneous PIPAAm polymer and its copolymer with butyl methacrylate (BMA) were synthesized and grafted to aminopropyl silica by activated ester-amine coupling and they were used as packing materials. The surface properties and functions of the stationary phases are controlled by external temperature. Isocratic elution by aqueous mobile phase alone is the basis for separation of peptides and protein. The separation of the mixture of three peptides, insulin chain A and B and β-endorphin fragment 1-27 was achieved by changing the column temperature with 0.9% NaCl aqueous solution as the sole eluent. Retention of peptides and proteins was controlled both by column temperature and by NaCl concentration in the aqueous mobile phases in this chromatographic system.
KW - Peptide
KW - Poly(N-isopropylacrylamide)
KW - Protein
KW - Temperature-responsive chromatography
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U2 - 10.1016/S0731-7085(96)02004-3
DO - 10.1016/S0731-7085(96)02004-3
M3 - Article
C2 - 9226588
AN - SCOPUS:0030923141
SN - 0731-7085
VL - 15
SP - 1545
EP - 1550
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 9-10
ER -