Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

Ni Putu Desy Aryantini; Daisuke Kondoh; Keita Nishiyama; Yuji Yamamoto; Takao Mukai; I. Nengah Sujaya; Tadasu Urashima; Kenji Fukuda

doi:10.1093/femsle/fnx056

Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

Ni Putu Desy Aryantini, Daisuke Kondoh, Keita Nishiyama, Yuji Yamamoto, Takao Mukai, I. Nengah Sujaya, Tadasu Urashima, Kenji Fukuda

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

Original language	English
Article number	fnx056
Journal	FEMS microbiology letters
Volume	364
Issue number	6
DOIs	https://doi.org/10.1093/femsle/fnx056
Publication status	Published - 2017 Mar 1
Externally published	Yes

Keywords

Cell surface proteins
Host-microbial interactions
Lactic acid bacteria
Laminin
Probiotics
Ribosomal proteins

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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title = "Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22",

abstract = "Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.",

keywords = "Cell surface proteins, Host-microbial interactions, Lactic acid bacteria, Laminin, Probiotics, Ribosomal proteins",

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doi = "10.1093/femsle/fnx056",

language = "English",

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T1 - Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

AU - Aryantini, Ni Putu Desy

AU - Kondoh, Daisuke

AU - Nishiyama, Keita

AU - Yamamoto, Yuji

AU - Mukai, Takao

AU - Sujaya, I. Nengah

AU - Urashima, Tadasu

AU - Fukuda, Kenji

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

AB - Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

KW - Cell surface proteins

KW - Host-microbial interactions

KW - Lactic acid bacteria

KW - Laminin

KW - Probiotics

KW - Ribosomal proteins

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Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

Abstract

Keywords

ASJC Scopus subject areas

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