Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum

Yasuo Hosaka, Atsushi Saito, Ryo Maeda, Chie Fukaya, Satoru Morikawa, Asako Makino, Kazuyuki Ishihara, Taneaki Nakagawa

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Objective: To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. Design: Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated. Results: In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration. Conclusion: These results suggest that 30 s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.

Original languageEnglish
Pages (from-to)364-368
Number of pages5
JournalArchives of Oral Biology
Issue number4
Publication statusPublished - 2012 Apr


  • Antibacterial agent
  • Biofilm
  • F. nucleatum
  • P. gingivalis
  • Periodontopathic bacteria
  • Povidone-iodine

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology


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