Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein

Haruka Sudo, Hong Jiang, Takashi Yasukawa, Yuichi Hashimoto, Takako Niikura, Masaoki Kawasumi, Shuji Matsuda, Yuji Takeuchi, Sadakazu Aiso, Masaaki Matsuoka, Yoshitake Murayama, Ikuo Nishimoto

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.

Original languageEnglish
Pages (from-to)708-723
Number of pages16
JournalMolecular and Cellular Neuroscience
Issue number6
Publication statusPublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology


Dive into the research topics of 'Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein'. Together they form a unique fingerprint.

Cite this