TY - JOUR
T1 - APC protein is required for initiation of neuronal differentiation in rat pheochromocytoma PC12 cells
AU - Dobashi, Yoh
AU - Katayama, Kazuhiro
AU - Kawai, Masataka
AU - Akiyama, Tetsu
AU - Kameya, Toru
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research, Nos. 11670191 and 12215141, from the Ministry of Education, Science, and Culture in Japan, and by Kitasato University Medical Science Research Project 2005.
PY - 2000/12/20
Y1 - 2000/12/20
N2 - The adenomatous polyposis (APC) gene product is highly expressed in the central nervous system. To elucidate the contribution of the APC protein to neuronal differentiation, we used an inducible antisense mRNA vector to suppress APC protein expression and examined neuronal differentiation of PC12 cells induced by nerve growth factor (NGF). When antisense mRNA was induced, APC protein expression was suppressed to 20% of the noninduced level. In those cells, neurite extension induced by NGF and expression of microtubule-associated protein 2 (MAP2) was completely inhibited. However, once cells had differentiated, antisense APC mRNA expression and subsequent suppression of APC protein expression had no effect on either cell morphology or MAP2 protein expression. These results suggest that the wild type APC is critically involved only in the initiation of neuronal differentiation, but not in the maintenance of the differentiated phenotype, or that the neuronal phenotype could be maintained at lower level of APC protein.
AB - The adenomatous polyposis (APC) gene product is highly expressed in the central nervous system. To elucidate the contribution of the APC protein to neuronal differentiation, we used an inducible antisense mRNA vector to suppress APC protein expression and examined neuronal differentiation of PC12 cells induced by nerve growth factor (NGF). When antisense mRNA was induced, APC protein expression was suppressed to 20% of the noninduced level. In those cells, neurite extension induced by NGF and expression of microtubule-associated protein 2 (MAP2) was completely inhibited. However, once cells had differentiated, antisense APC mRNA expression and subsequent suppression of APC protein expression had no effect on either cell morphology or MAP2 protein expression. These results suggest that the wild type APC is critically involved only in the initiation of neuronal differentiation, but not in the maintenance of the differentiated phenotype, or that the neuronal phenotype could be maintained at lower level of APC protein.
KW - APC
KW - Antisense
KW - Inducible vector
KW - Neuronal differentiation
KW - PC12 cells
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U2 - 10.1006/bbrc.2000.4015
DO - 10.1006/bbrc.2000.4015
M3 - Article
C2 - 11118346
AN - SCOPUS:0034694832
SN - 0006-291X
VL - 279
SP - 685
EP - 691
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -