TY - JOUR
T1 - Application of cationic latex particles for protein separation
AU - Sumi, Yoshiteru
AU - Shiroya, Toshifumi
AU - Fujimoto, Keiji
AU - Wada, Tadashi
AU - Handa, Hiroshi
AU - Kawaguchi, Haruma
N1 - Funding Information:
This work was supported by the HFSP grant and by a Grant-in-Aid for scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan (04239102).
PY - 1994/5/9
Y1 - 1994/5/9
N2 - We have prepared cationic latex particles with amino groups on their surface. The particles were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer, to which hexamethylenediamine was immobilized by means of coupling with epoxy groups. The zeta potential of the particles was approximately 30 mV and the isoelectric point was 10.5. The latex particles preferentially adsorbed acidic proteins through an electrostatic interaction. We compared the capacity and selectivity in adsorption of acidic proteins on the cationic particles and on diethylaminoethyl (DEAE)-Sepharose gel, which has been conventionally used. The latex particles were twenty times more efficient in adsorption of proteins than DEAE-Sepharose gel. Acidic proteins encoded by the adenovirus Ela gene were selectively separated from crude cell extracts using the cationic latex particles.
AB - We have prepared cationic latex particles with amino groups on their surface. The particles were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer, to which hexamethylenediamine was immobilized by means of coupling with epoxy groups. The zeta potential of the particles was approximately 30 mV and the isoelectric point was 10.5. The latex particles preferentially adsorbed acidic proteins through an electrostatic interaction. We compared the capacity and selectivity in adsorption of acidic proteins on the cationic particles and on diethylaminoethyl (DEAE)-Sepharose gel, which has been conventionally used. The latex particles were twenty times more efficient in adsorption of proteins than DEAE-Sepharose gel. Acidic proteins encoded by the adenovirus Ela gene were selectively separated from crude cell extracts using the cationic latex particles.
KW - Cationic latex particles
KW - Protein separation
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U2 - 10.1016/0927-7765(94)80006-5
DO - 10.1016/0927-7765(94)80006-5
M3 - Article
AN - SCOPUS:0028769199
SN - 0927-7765
VL - 2
SP - 419
EP - 427
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 4
ER -