TY - JOUR
T1 - Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells
AU - Watanabe, Sachiko
AU - Moniaga, Catharina Sagita
AU - Nielsen, Søren
AU - Hara-Chikuma, Mariko
N1 - Funding Information:
This work was supported by grants from Astellas Pharma Inc. in the Creation of Innovation Centers for Advanced Inter disciplinary Research Areas Program, the Ministry of Education, Culture, Sports, Science, and Technology of Japan Grant Number 24591648 (M.H-C.), The Tokyo Biochemical Research Foundation (C.S.M. and M.H-C.) .
Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016/2/26
Y1 - 2016/2/26
N2 - Aquaporin (AQP) 9, a member of the transmembrane water channel family, is defined as a water/glycerol transporting protein. Some AQPs including AQP3 and AQP8 have been recently found to transport hydrogen peroxide (H2O2). Here we show that AQP9 facilitates the membrane transport of H2O2 in human and mice cells. Enforced expression of human AQP9 in Chinese hamster ovary-K1 potentiated the increase in cellular H2O2 after adding exogenous H2O2. In contrast, AQP9 knockdown by siRNA in human hepatoma HepG2 cells reduced the import of extracellular H2O2. In addition, the uptake of extracellular H2O2 was suppressed in erythrocytes and bone marrow-derived mast cells from AQP9 knockout mice compared with wild-type cells. Coincidentally, H2O2-induced cytotoxicity was attenuated by AQP9 deficiency in human and mice cells. Our findings implicate the involvement of AQP9 in H2O2 transport in human and mice cells.
AB - Aquaporin (AQP) 9, a member of the transmembrane water channel family, is defined as a water/glycerol transporting protein. Some AQPs including AQP3 and AQP8 have been recently found to transport hydrogen peroxide (H2O2). Here we show that AQP9 facilitates the membrane transport of H2O2 in human and mice cells. Enforced expression of human AQP9 in Chinese hamster ovary-K1 potentiated the increase in cellular H2O2 after adding exogenous H2O2. In contrast, AQP9 knockdown by siRNA in human hepatoma HepG2 cells reduced the import of extracellular H2O2. In addition, the uptake of extracellular H2O2 was suppressed in erythrocytes and bone marrow-derived mast cells from AQP9 knockout mice compared with wild-type cells. Coincidentally, H2O2-induced cytotoxicity was attenuated by AQP9 deficiency in human and mice cells. Our findings implicate the involvement of AQP9 in H2O2 transport in human and mice cells.
KW - Aquaporin 9
KW - Hydrogen peroxide
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U2 - 10.1016/j.bbrc.2016.01.153
DO - 10.1016/j.bbrc.2016.01.153
M3 - Article
C2 - 26837049
AN - SCOPUS:84958902982
SN - 0006-291X
VL - 471
SP - 191
EP - 197
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -