@article{a03cc8aeb78e46e3960c1a38cb2b7472,
title = "Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation",
abstract = "Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.",
keywords = "Armitage, Gasz, Yb body, mitochondrion, piRNA",
author = "Haruna Yamashiro and Mayu Negishi and Tatsuki Kinoshita and Hirotsugu Ishizu and Hitoshi Ohtani and Siomi, {Mikiko C.}",
note = "Funding Information: We thank S. Hirakata and K. Saito for comments on this manuscript and technical support, respectively. We also thank all other members of the Siomi laboratory at the University of Tokyo. This study was supported by research grants from MEXT for M.C.S. (19H05466) and H.I. H.Y. is supported by the Japan Society for the Promotion of Science. T.K. is supported by the Graduate Program for Leaders in Life Innovation. Funding Information: We thank S. Hirakata and K. Saito for comments on this manuscript and technical support, respectively. We also thank all other members of the Siomi laboratory at the University of Tokyo. This study was supported by research grants from MEXT for M.C.S. (19H05466) and H.I. H.Y. is supported by the Japan Society for the Promotion of Science. T.K. is supported by the Graduate Program for Leaders in Life Innovation. Publisher Copyright: {\textcopyright} 2019 The Authors",
year = "2020",
month = feb,
day = "5",
doi = "10.15252/embr.201948769",
language = "English",
volume = "21",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Nature Publishing Group",
number = "2",
}