Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Takuya Terai; Moe Kohno; Gaelle Boncompain; Shigeru Sugiyama; Nae Saito; Ryo Fujikake; Tasuku Ueno; Toru Komatsu; Kenjiro Hanaoka; Takayoshi Okabe; Yasuteru Urano; Franck Perez; Tetsuo Nagano

doi:10.1021/jacs.5b05672

Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Takuya Terai
, Moe Kohno
, Gaelle Boncompain
, Shigeru Sugiyama
, Nae Saito
, Ryo Fujikake
, Tasuku Ueno
, Toru Komatsu
, Kenjiro Hanaoka
, Takayoshi Okabe
, Yasuteru Urano
, Franck Perez
, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Artificial ligands of streptavidin (ALiS) with association constants of 10⁶ M^-1 were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

Original language	English
Pages (from-to)	10464-10467
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	137
Issue number	33
DOIs	https://doi.org/10.1021/jacs.5b05672
Publication status	Published - 2015 Aug 11
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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Terai, T., Kohno, M., Boncompain, G., Sugiyama, S., Saito, N., Fujikake, R., Ueno, T., Komatsu, T., Hanaoka, K., Okabe, T., Urano, Y., Perez, F., & Nagano, T. (2015). Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport. Journal of the American Chemical Society, 137(33), 10464-10467. https://doi.org/10.1021/jacs.5b05672

Terai, T, Kohno, M, Boncompain, G, Sugiyama, S, Saito, N, Fujikake, R, Ueno, T, Komatsu, T, Hanaoka, K, Okabe, T, Urano, Y, Perez, F & Nagano, T 2015, 'Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport', Journal of the American Chemical Society, vol. 137, no. 33, pp. 10464-10467. https://doi.org/10.1021/jacs.5b05672

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abstract = "Artificial ligands of streptavidin (ALiS) with association constants of 106 M-1 were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.",

author = "Takuya Terai and Moe Kohno and Gaelle Boncompain and Shigeru Sugiyama and Nae Saito and Ryo Fujikake and Tasuku Ueno and Toru Komatsu and Kenjiro Hanaoka and Takayoshi Okabe and Yasuteru Urano and Franck Perez and Tetsuo Nagano",

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doi = "10.1021/jacs.5b05672",

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AU - Terai, Takuya

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AU - Boncompain, Gaelle

AU - Sugiyama, Shigeru

AU - Saito, Nae

AU - Fujikake, Ryo

AU - Ueno, Tasuku

AU - Komatsu, Toru

AU - Hanaoka, Kenjiro

AU - Okabe, Takayoshi

AU - Urano, Yasuteru

AU - Perez, Franck

AU - Nagano, Tetsuo

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AB - Artificial ligands of streptavidin (ALiS) with association constants of 106 M-1 were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

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Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Abstract

ASJC Scopus subject areas

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